| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,738,402 | 0 | A | C | 52.6% | 5.7 / 12.4 | 19 | D80A (GAC→GCC) | yiaN | 2,3‑diketo‑L‑gulonate transporter large permease YiaN |
| Reads supporting (aligned to +/- strand): ref base A (3/6); new base C (0/10); total (3/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.67e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.27e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGA‑TGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTT > NZ_CP009273/3738325‑3738485 | ctcCCTGCTGGCGATTCCGTTTTTTGTGCTGGGGGGTGAAATCATGAATGCGGGGGGCTTGTCAAAACGCTTTGTTGCCCTGCCGA‑TGaa < 1:468481/90‑1 (MQ=255)ctcCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGA‑TGaa < 2:3596/90‑1 (MQ=255) cccTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGGGGCTTGTCAAAACGCATTTTTTACCTGCCGG‑TGAAAc > 2:645645/1‑90 (MQ=255) tgctgGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATCAATACCGGCGGCGTTCCAAAACCCGTTGTTGAGCTGCCGACTGaaaggg > 1:574818/1‑87 (MQ=255) ctgGCTTTTCCGTTCTTTGGGGGGGGGGGTGAAATCATGAGGGGGGGGGGCTTGACAAAACGCATTGTTGCCCTGCCGA‑TGAAACtggtg < 1:432365/90‑1 (MQ=255) ctgGCGATTCCTTTTTTTGTGCTGGCGGGTGAAATCATGAATGGGGGGGGCTTGTAAAAACGCATTGTTGCCCTGCCGA‑TGAAACtggtg < 1:87798/90‑1 (MQ=255) ctgGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTAAAAACGCATTGTTGACCTGCCGA‑TGAAACtggtg < 2:654185/90‑1 (MQ=255) tgGCGATTCCTTTTTTTGTGCTGGGGGGTGAAATCATGAATGGGGGGGGCTTGTCAAAACGCATTGTTGCCCTGCCGA‑TGAAACtggtgg < 1:250434/90‑1 (MQ=255) tgGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGGGGCTTGTCAAAACGCATTGTTGACCTGCCGA‑TGAAACtggtgg < 2:388924/90‑1 (MQ=255) tgGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGA‑TGAAACtggtgg < 2:138370/90‑1 (MQ=255) aTTCCGTTCTTTGTGCTGGGGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGA‑TGAAACTGGTGGGGCAt < 2:692430/90‑1 (MQ=255) gtgCTGGCGGGTGAAATCATGAATGCGGGGGGTTTGTCAAAACGCATTGTTGCCCTGCCGA‑TGAAACTGGT‑GGGCATAAACCAGGCgggc < 2:652297/90‑1 (MQ=255) tgCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGA‑TGAAACTGGTGGGGCATAAACCAGGCgggc < 2:293297/90‑1 (MQ=255) ggCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGA‑TGAAACTGGTGGGGCATAAACCAGGCgggctggg > 2:103675/1‑90 (MQ=255) taaaaTCATGAAGGCGGGGGGTTTGTAAAAACGCTTTGTTGCCCTGCCGA‑TGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGt < 1:230255/88‑1 (MQ=255) tggggggggTTTGACAAAACGCTTTGTTGCCCTGCCGA‑TGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGc < 1:179263/87‑1 (MQ=255) tgggggcggCTTGTCAAAACGCATTGTTGCCCTGCCGA‑TGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGc < 2:561934/87‑1 (MQ=255) accGCTTTGTTGCCCTGCCGA‑TGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGa < 1:444347/88‑1 (MQ=255) ttgttgCCCTGCCGA‑TGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCttt < 1:645645/90‑1 (MQ=255) | CTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGA‑TGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTT > NZ_CP009273/3738325‑3738485 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |