Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	49,884	C→A	P21Q (CCG→CAG)	folA →	type 3 dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	49,884	0	C	A	100.0%	29.2 / NA	10	P21Q (CCG→CAG)	folA	type 3 dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (6/4);  total (6/4)

TCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTT  >  NZ_CP009273/49820‑49929                                                                 |                                              tcAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGcc                      <  2:599569/90‑1 (MQ=255)           gTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAAc            >  2:215844/1‑90 (MQ=255)               gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCaa        >  1:377115/1‑90 (MQ=255)               gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCaa        <  2:660772/90‑1 (MQ=255)                    cggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCt   <  2:631658/90‑1 (MQ=255)                     ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCtt  >  1:180483/1‑90 (MQ=255)                     ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCtt  >  2:600151/1‑90 (MQ=255)                     ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGGGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCtt  >  1:606916/1‑90 (MQ=255)                                         ttATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCaa        >  1:545272/1‑64 (MQ=255)                                         ttATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCaa        <  2:545272/64‑1 (MQ=255)                                                                 |                                              TCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTT  >  NZ_CP009273/49820‑49929

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑