Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,632,461	T→G	L487R (CTT→CGT)	pitA →	inorganic phosphate transporter PitA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,632,461	0	T	G	100.0%	94.9 / NA	26	L487R (CTT→CGT)	pitA	inorganic phosphate transporter PitA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (17/9);  total (17/9)

GTAGATGGTGGCGGCTTACAGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGAT  >  NZ_CP009273/3632379‑3632544                                                                                   |                                                                                    gtAGATGGTGGCGGCTTACAGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCcggc                                                                              >  1:707424/1‑90 (MQ=255)      tggtggCGGCTTACAGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGCt                                                                         >  1:204472/1‑90 (MQ=255)        gtggCGGCTTACAGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTGt                                                                       >  2:102823/1‑90 (MQ=255)        gtggCGGCTTACAGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTGt                                                                       >  1:610559/1‑90 (MQ=255)             ggcTTACAGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTgg                                                                  >  2:146753/1‑90 (MQ=255)                       gTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGc                                                        >  1:296323/1‑90 (MQ=255)                             ccGTTACCAGCATTCTGCTGGCCTGGGTCTTTACCCTTCCGGCTGCGGTACTTCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTcc                                                  <  2:308009/90‑1 (MQ=255)                               gTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTg                                                >  2:233306/1‑90 (MQ=255)                                                ggCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCaaa                               >  2:180183/1‑90 (MQ=255)                                                   cTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACg                            >  1:381838/1‑90 (MQ=255)                                                     gggTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGc                                                                          >  2:655544/1‑42 (MQ=255)                                                     gggTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGc                                                                          <  1:655544/42‑1 (MQ=255)                                                     gggTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTAc                                       >  2:169448/1‑77 (MQ=255)                                                     gggTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTAc                                       <  1:169448/77‑1 (MQ=255)                                                     gggTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAg                          >  1:549493/1‑90 (MQ=255)                                                           ttACCCTTCCGGCTGCGGTACTGCGATCCGGCGGGCTGCACTGGCTCCCCTTGCAGTTCCTGCAATCGTAAGCACCAAAACGAGCGGGTc                    >  2:428037/1‑90 (MQ=255)                                                               ccTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCt                <  1:280085/90‑1 (MQ=255)                                                                cTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTg               >  1:307824/1‑90 (MQ=255)                                                                 ttCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTgg              >  1:516588/1‑90 (MQ=255)                                                                 ttCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTgg              >  2:134353/1‑90 (MQ=255)                                                                 ttCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTgg              >  2:360314/1‑90 (MQ=255)                                                                   ccGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGcc            <  1:366999/90‑1 (MQ=255)                                                                           ggTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAg    <  1:245153/90‑1 (MQ=255)                                                                           ggTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAg    <  2:28382/90‑1 (MQ=255)                                                                             tACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGAt  <  2:561325/90‑1 (MQ=255)                                                                             tACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGAt  <  1:146753/90‑1 (MQ=255)                                                                                   |                                                                                    GTAGATGGTGGCGGCTTACAGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGAT  >  NZ_CP009273/3632379‑3632544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑