| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 546,689 | 0 | T | G | 56.2% | 12.2 / 18.9 | 16 | S265R (AGT→AGG) | ybcF | carbamate kinase |
| Reads supporting (aligned to +/- strand): ref base T (0/7); new base G (9/0); total (9/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.74e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.80e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACGTGCCATTCGCCATGCCACACCGGATGAGTTAGCGCCATTTGCCAAAGCCGATGGTTCGATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACC > NZ_CP009273/546602‑546773 | aCGTGCCATTCGCCATGCCACACCGGATGAGTTAGCGCCATTTGCCAAAGCCGATGGTTCGATGGGGCCGAATGTAACGGCGGGGAgggg > 1:158033/1‑90 (MQ=255) gTGCCATTCGCCATGCCACACCGGATGAGTTAGCGCCATTTGCCAAAGCCGATGGTTCGATGGGGCCGAATGTAACGGCGGTGAGGGGtt > 1:753622/1‑90 (MQ=255) gTGCCATTCGCCACGCCACACCGGATGAGTTAGCGCCATTTGCCAAAGCCGATGGTTCGATGGGGCCGAATGTAACGGCGGTGAGTGGtt < 1:707841/90‑1 (MQ=255) ccATGCCACACCGGATGAGTTAGCGCCATTTGCCAAAGCCGATGGTTCGATGGGGCCGAATGTAACGGCGGGGGGGGGGTTTGTTAGAAg > 1:464872/1‑90 (MQ=255) aTGCCACACCGGATGAGTTAGCGCCGTTTGCCAAAGCCGATGGTTCGATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGcc < 2:65222/90‑1 (MQ=255) ggATGAGTTAGCGCCATTTGCCAAAGCCGATGGTTCGATGGGGCCGAATGTAACGGCGGGGGGGGGGGGTGTTGGAAGCCGCGGGAAAcc > 1:781980/1‑90 (MQ=255) aGTTAGCGCCATTTGCCAAAGCCGATGGTTCGATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGt < 1:543588/90‑1 (MQ=255) gcgcCATTTGCCAAAGCCGATGGTTCGATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGAtt < 1:232272/90‑1 (MQ=255) gcgcCATTTGCCAAAGCCGATGGTTCGATGGGGCCGAATGTAACGGCGGGGGGGGGTTTTTTGAGAAGCCCCGGTAAAACCCCGCGGGtt > 2:717971/1‑90 (MQ=255) aTTTGCCAAAGCCGATGGTTCGATGGGGCTGAATGTAACGGCGGTGAGGGGGTATGTCAGAAGCCGTGGTAAAACCGCGTGGGTTggggg > 2:251761/1‑89 (MQ=255) aTTTGCCAAAGCCGATGGTTCGATGGGGCCGAATGTAACGGCGGTGGGGGGTTATGTGAGAAGCCGTGGTGAAACCGCGGGGGGTGGGGc > 2:232272/1‑90 (MQ=255) tttGCCAAAGCCGATGGTTCGATGGGGCCGAATGTAACGGCGGTGGGGGGGTTTGTCAGAAACCGGGGTAAAACCCCGGGGGTTtggggg > 2:157178/1‑88 (MQ=255) gATGGTTCGATGGGGCCGAATGTAACGGCGGTGGGGGGGTATGTCAGAAGCCGTGGGAAACCCCCGGGGGTTGGGGGGGTTTTGCGaaat > 2:352888/1‑88 (MQ=255) gATGGTTCGATGGGGCCGAATGTAACGGCGGTGAGGGGGTATGTCAGAAGCCGTGGGAAACCCCCGTGGGTTGGGGGGGTATCGCGAAtt > 2:66606/1‑90 (MQ=255) gcggTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCgggg < 2:753622/90‑1 (MQ=255) gTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGAcc < 1:157178/90‑1 (MQ=255) gTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGAcc < 1:165927/90‑1 (MQ=255) | ACGTGCCATTCGCCATGCCACACCGGATGAGTTAGCGCCATTTGCCAAAGCCGATGGTTCGATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACC > NZ_CP009273/546602‑546773 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |