| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,267,278 | 0 | T | G | 54.5% | 7.6 / 12.8 | 11 | T10P (ACC→CCC) | chaA | sodium‑potassium/proton antiporter ChaA |
| Reads supporting (aligned to +/- strand): ref base T (0/5); new base G (6/0); total (6/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.16e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGCGGGTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTA‑TCCCTTTGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAA > NZ_CP009273/1267196‑1267362 | ggTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGCGGGgttttttc > 2:1586/1‑87 (MQ=255) gTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGCGGGTTTTTACCg < 2:409029/90‑1 (MQ=255) aGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGCGGGTTTTTACCGcc < 2:197216/90‑1 (MQ=255) tgtCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGCGGGGTTTTTTCGcccc > 1:375263/1‑88 (MQ=255) ctTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGCGGGGTTTTTCCCCCCCTTTTGCAttt > 2:509047/1‑90 (MQ=255) acaGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGCGGGTTTTTACCGCCTCTTGAGCATTTGACATTa < 1:772884/90‑1 (MQ=255) ggAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGCGGGGTTTTTACGCCCCTTGGGGAATTTACAtttttg > 2:772884/1‑86 (MQ=255) tACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGCGGGTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTA‑TCCCttt < 1:290753/90‑1 (MQ=255) aGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGCGGGGTTTTTTCCCCCCTTTTGCATTTTTCATTATGGTTATTCCCttttcg > 2:441777/1‑87 (MQ=255) cgcCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGCGGGGTTTTTCCGCCCCCTGTGCATTTTTCATTATGGTTT‑TTCCTTTTCAggt > 2:799022/1‑88 (MQ=255) cgcCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGCGGGGTTTTTCCCCCCCTTTAGCATTTTACATTTTGGTTT‑TCCCTTTTCAGAt > 2:650063/1‑90 (MQ=255) gCGGGTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTA‑TCCCTTTGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGaaaaa < 1:107231/90‑1 (MQ=255) | GGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGCGGGTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTA‑TCCCTTTGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAA > NZ_CP009273/1267196‑1267362 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |