| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,698,298 | 0 | G | T | 53.8% | 5.2 / 21.3 | 13 | H90N (CAT→AAT) | ydgJ | oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base G (6/0); new base T (0/7); total (6/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.83e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.56e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATC‑AACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTA > NZ_CP009273/1698210‑1698386 | gacagCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATC‑AACGACCACATGt > 1:548369/1‑90 (MQ=255) agCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATC‑AACGACCACATGTTTa > 1:604307/1‑90 (MQ=255) cacGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATC‑AACGACCACATGTTTACCCg > 2:681611/1‑90 (MQ=255) cAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATC‑AACGACCACATGTTTACCCGCCTCAAg > 2:564916/1‑90 (MQ=255) tttGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATC‑AACGACCACATGTTTACCCGCCTCAAGCGCCGCt > 2:695394/1‑90 (MQ=255) ccctctCGCTCTTCTGCCGGTGCCAGGGAAAGGGTTTTAAC‑AACGCCCCTTTTTTTCCCCGCCTCAAGCGCCGCTTTGGCTAACGGGaaa < 1:516016/88‑1 (MQ=255) aGCTCCCGCGTTTGTGACGGTGTCGGGGTAAGGGTTTTACA‑AACGCCCACTTTTTTCCCCGCCTCAAGCGCCGCTTTGGCTAACGGGaaa < 1:804413/90‑1 (MQ=255) tgggACAGTGTCAGGGTAAGGGTTTTAAC‑AACCACCACTTTTTTCCCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTg < 1:564916/87‑1 (MQ=255) aGGGTTTAACAAACGCCCACTTTTTTCCCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATc < 1:227427/90‑1 (MQ=255) tttATC‑AACCCCCACATTTTTGCCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTc < 2:224982/90‑1 (MQ=255) c‑AACGCCCATTTTTTTCCCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGt < 2:548369/90‑1 (MQ=255) gcccACATTTTTCCCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTggg < 2:47736/88‑1 (MQ=255) tGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTa > 2:111747/1‑90 (MQ=255) | GACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATC‑AACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTA > NZ_CP009273/1698210‑1698386 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |