| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,175,960 | 0 | G | T | 54.5% | 17.3 / 31.9 | 22 | G18C (GGC→TGC) | zupT | zinc transporter ZupT |
| Reads supporting (aligned to +/- strand): ref base G (4/6); new base T (12/0); total (16/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.81e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCA‑CGTTTATTGGCGCGTTTCTCGGCGTTCTC‑GGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATC > NZ_CP009273/3175874‑3176049 | tCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGGGGCC‑CGGTTATTgtgc > 1:508989/1‑87 (MQ=255) cTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCA‑CGTTTATTGgcgc < 2:124002/90‑1 (MQ=255) gAAGGTGAAGATTTACAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGGGCCA‑CGCTTTTTTGGGCGgtttt > 1:622498/1‑88 (MQ=255) gTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGAGCCC‑CGCTTTTTTGGGCGGTTTTCGGCGTTCTC‑GGGCAAAAAcc > 2:377717/1‑90 (MQ=255) gTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCA‑CGTTTTTTGGCGCGTTTTTCGGCGTTCTC‑CGGCAAAAAcc > 2:151730/1‑90 (MQ=255) tGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGGGCCA‑CGTTTTTTTGGGCGGTTTTCCGCGTTCTCTGGGGAAAAACCC‑CGAAcc > 1:592784/1‑90 (MQ=255) tGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCC‑CGGTTTTTTGCGCGTTTTTCCGCGTTTTC‑GGGCAAAAAACCCCCAAcc > 2:676890/1‑90 (MQ=255) gTACCTCTCATTCTGACCATACTGGCGGGGGCGGCCC‑CGGTTTTTTGCGCGGTTTTCCGCGTTCTC‑GGGCAAAAAACCCCGAACCGCTTa > 1:30385/1‑90 (MQ=255) tctcATTCTGACCATACTGGCGGGGGGGGCCC‑CGTTTTTTTGCGCGGTTCTCGGCGTTCTC‑GGGCAAAAAACCCCGAACCGCCTTCTggg > 1:256350/1‑89 (MQ=255) tctcATTCTGACCATACTGGCGGGGGGAGCCC‑CGTTTTTTTGCGCGGTTTTCGGCGTTCTC‑CGGCAAAAAACCCCGAACCCCTTACTGGc > 2:592041/1‑90 (MQ=255) tctcATTCTGACCATACTGGCGGGGGCAGCCA‑CGTTTTTTTGCGCGTTTCTCGGCGTTCTC‑GGGCAAAAAACCCCGAACCGCTTACTGGc > 2:264175/1‑90 (MQ=255) tctcATTCTGACCATACTGGCGGGGGCAGCCA‑CCTTTTTTTGGGCGGTTTTCCGCCTTCTC‑GGGCAAAAACCCCCGAACCCCCTACTggg > 2:730683/1‑89 (MQ=255) cTGACCATACTGGCGGGGGCAGCCC‑CGGTTTTTTGCGCGTTTTTCGGCGTTCTCTGGGCAAAAACCC‑CCAACCGCTTACTGGCGgttttg > 1:626483/1‑88 (MQ=255) aCCATACTGGCGGGGGCAGCCA‑CGTTTATTGGCGCGTTTCTCGGCGTTCTC‑GGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTa < 2:637371/90‑1 (MQ=255) tACTGGCGGGGGCAGCCA‑CGTTTTTTTGCGCGTTTTTCGGCGTTTTC‑GGGCAAAAACCCCCCAACCCCCTACTGGCGGTTTCGCTAgggt > 2:663822/1‑88 (MQ=255) tACTGGCGGGGGCAGCCA‑CGTTTATTTGCGCGGTTTTCGGCGTTCTC‑GGGCAAAAAACCCCGAACCGCTTTCTGGGGGTTTTGCCAGGtt > 2:745455/1‑90 (MQ=255) gCGGGGGGAGCCACCGTTTTTTGG‑GCGTTTCTCGGCGGTCTC‑CGGGAAAAAACCCCCAACCCCCTTCTTGCGGTTTTGCTAGGGTTTtcg > 1:174751/1‑90 (MQ=255) ggggCAGCCA‑CGTTTATTGGCGCGTTTCTCGGCGTTCTC‑GGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTgcggcg < 2:554611/90‑1 (MQ=255) gCAGCCA‑CGTTTATTGGCGCGTTTCTCGGCGTTCTC‑GGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCgggg < 2:662235/90‑1 (MQ=255) aGCCA‑CGTTTATTGGCGCGTTTCTCGGCGTTCTC‑GGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGAt < 1:377717/90‑1 (MQ=255) cGTTTATTGGCGCGTTTCTCGGCGTTCTC‑GGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGt < 1:151730/90‑1 (MQ=255) ttGGCGCGTTTCTCGGCGTTCTC‑GGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGGGCATGTTGctcc > 1:249481/1‑89 (MQ=255) ttGGCGCGTTTCTCGGCGTTCTC‑GGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGctca > 2:283860/1‑90 (MQ=255) ggCGCGTTTCTCGGCGTTCTC‑GGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGctcatc < 2:119154/90‑1 (MQ=255) ggCGCGTTTCTCGGCGTTCTC‑GGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGctcatc < 1:213397/90‑1 (MQ=255) ggCGCGTTTCCCGGCGTTCTC‑GGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGctcatc < 2:347305/90‑1 (MQ=255) | TCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCA‑CGTTTATTGGCGCGTTTCTCGGCGTTCTC‑GGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATC > NZ_CP009273/3175874‑3176049 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |