| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,058,958 | 0 | G | A | 61.1% | ‑0.5 / 16.6 | 18 | L57L (CTG→TTG) | yihO | MFS transporter |
| Reads supporting (aligned to +/- strand): ref base G (4/3); new base A (0/11); total (4/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.14e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.86e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGA > NZ_CP009273/4058870‑4059038 | aaTTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGa < 2:74542/90‑1 (MQ=255)aaTTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGa < 1:396232/90‑1 (MQ=255) cccaaaTTTTTTCCCGGGAGTCAAAAAAAAACCGGGGGAACATACCGGAAAACGCGGAAAAAAACTTCGCCACAAAAAAAAAAATCCCGc < 1:76033/86‑1 (MQ=255) ccGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAgg > 1:175164/1‑90 (MQ=255) aaaCCCGGTGAGCATATGGGAAAACGCGGAAAAAACCTCCCCCACCAAAAAAATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATc < 2:710795/90‑1 (MQ=255) aaTCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCCCCACCAAAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCg < 2:133920/90‑1 (MQ=255) ggTGAGCATATCGAAAAACGGGGAAAAAACCTCCCCCACAAAAAAAAAAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGtata < 1:116559/90‑1 (MQ=255) tatCGGAAAACGCGGAAAAAAACTTCGCCACCAAAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTga < 2:328207/90‑1 (MQ=255) tatCGGAAAACGCGAAAAAAACCTCCCCCACAAAAAAAAAAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTga < 1:264387/90‑1 (MQ=255) atCGGAAAACGGGAAAAAAACCTCCCCCACAAAAAAAAAAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTgag < 1:22858/90‑1 (MQ=255) cGGTAAAGGGGGAAAAAATCTCCCCCACAAAAAAAAAAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTgagga < 1:99012/90‑1 (MQ=255) cGGAAAACGCGGAAAAAACCTTCCCCACCAAAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTgagga < 2:182245/90‑1 (MQ=255) cGGAAAACGCGGAAAAAACCTCCCCCACAAAAAAAAAAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTgagga < 1:502529/90‑1 (MQ=255) aaaCGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCTGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATa > 1:297519/1‑90 (MQ=255) aaaCGCGGAAAAAAACTTCGCCCCAAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATa < 2:71856/90‑1 (MQ=255) accgcgGAAAAAACCTCCCCCCCCAAAAAAATAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATAc < 2:764451/88‑1 (MQ=255) gTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTa > 1:373348/1‑90 (MQ=255) tCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCaga > 1:273776/1‑90 (MQ=255) | AATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGA > NZ_CP009273/4058870‑4059038 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |