| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 368,096 | 0 | T | G | 53.8% | 11.5 / 16.4 | 13 | L176V (TTA→GTA) | mhpD | 2‑keto‑4‑pentenoate hydratase |
| Reads supporting (aligned to +/- strand): ref base T (1/5); new base G (7/0); total (8/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.66e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.56e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCT > NZ_CP009273/368011‑368184 | gTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGa < 1:621524/90‑1 (MQ=255) tCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGACCTGAAAAACTgcg > 2:445991/1‑90 (MQ=255) tGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCCGGGCCACGTCCCGCGGGGGTTGACCTGAAAAAACTCGCCATGAAGa > 1:426320/1‑90 (MQ=255) cAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCCCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGa < 2:311958/90‑1 (MQ=255) aTAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGAcgc < 2:588220/90‑1 (MQ=255) gCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGtaat < 2:549039/90‑1 (MQ=255) tCATCGGCGGGCCGGCGCAACGTCCGGCGGGGGTAGACCTGAAAAAATGCGCCCTGAAGAAGACGCGGAATAACGAAGAGGGTTTTAGCg > 1:389894/1‑90 (MQ=255) cATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTTGACCTGAAAAACTGCGCCCTGAAGAAGACGCGGAATAACAAAGAGGGTTCTAGCgg > 1:400562/1‑90 (MQ=255) aTCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGACCTGAAAAACTGCGCCATGAAGATGACGCGGAATAACGAAGAGGGTTTTAGCggg > 1:21178/1‑90 (MQ=255) tCGGCGGTCCGGCGCAACGTCCGGCGGGGGTTGACCTGAAAAACTGCGCCCTGAAGAAGACGCGGAAAAAAGAAGAGGGTTTTTGCgggg > 1:53201/1‑89 (MQ=255) tCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGACCTGAAAAAATGCGCCATGAAGATGACGCGTAATAACGAAGAGGGTTCTAGCGGgc > 1:28831/1‑90 (MQ=255) cgcAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAAt < 2:5890/90‑1 (MQ=255) gTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCt > 2:404792/1‑90 (MQ=255) | GTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCT > NZ_CP009273/368011‑368184 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |