Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273536,0140TG54.5% 5.9 / 12.4 11intergenic (+50/‑7)allB/ybbYallantoinase AllB/uracil/xanthine transporter
Reads supporting (aligned to +/- strand):  ref base T (1/4);  new base G (6/0);  total (7/4)
Fisher's exact test for biased strand distribution p-value = 1.52e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGTTG  >  NZ_CP009273/535949‑536099
                                                                 |                                                                                     
tAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTTTCCGGTTAAGGGGGGTTTATGTTCAATTTTGCAGTc                                                               >  2:205223/1‑90 (MQ=255)
      tCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTTTCCGGTTTAGGGGGGTTTTTGTTCAATTTTTCAGTCcgccgc                                                         >  2:609933/1‑90 (MQ=255)
      tCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGGTAAGGGGTGTTTTTGTTCAATTTTGCAGTCAGCcgc                                                         >  2:58764/1‑90 (MQ=255)
                    gCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATc                                           <  2:167270/90‑1 (MQ=255)
                          gCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGgtgt                                                                                     <  1:292117/42‑1 (MQ=255)
                          gCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGgtgt                                                                                     >  2:292117/1‑42 (MQ=255)
                                    tCCTTGCGGCGGGCATTCTCCGGGTAAGGGGTGTTTTTGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTTTTAGGATTTTAGTGGttt                           >  1:83485/1‑90 (MQ=255)
                                    tCCTTGCGGCGGGCATTCTCCGGGTAAGGGGGGTTTTTGTTCAATTTTTCAGTCAGCCCCGAAAGCCTGTTATCAGGATTTCAGTGGgtt                           >  2:278377/1‑90 (MQ=255)
                                        tGCGGCGGGCATTTTCCGGTTAAGGGGTGTTTATGTTCAATTTTTCAGTCAGCCGCGAAAGCCTGTTATCAGGGTTTTAGTGGttttttt                       >  2:360926/1‑88 (MQ=255)
                                                   ttCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGc            <  2:541646/90‑1 (MQ=255)
                                                        cGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAacac       <  1:260110/90‑1 (MQ=255)
                                                        cGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAacac       <  1:602071/90‑1 (MQ=255)
                                                             aaGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGTtg  >  2:355501/1‑90 (MQ=255)
                                                                 |                                                                                     
TAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGTTG  >  NZ_CP009273/535949‑536099

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑