Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273612,0120TG58.3% 9.1 / 12.3 12G800G (GGT→GGG) entFenterobactin non‑ribosomal peptide synthetase EntF
Reads supporting (aligned to +/- strand):  ref base T (0/5);  new base G (7/0);  total (7/5)
Fisher's exact test for biased strand distribution p-value = 1.26e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.93e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGTGATCTCTATCTCACTGGCATTCAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGAT  >  NZ_CP009273/611948‑612093
                                                                |                                                                                 
tATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGGGGTCTCTTTCTTACCGGCATTCaaa                                                          >  2:40663/1‑89 (MQ=255)
      aTACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGTGATCTCTATCTCACTGGCATTCAACTGgcgc                                                    <  1:439106/90‑1 (MQ=255)
        aCGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGGGGGCTCTTTCTCCCTGGCATTCAAATGgcgccg                                                  >  2:394955/1‑88 (MQ=255)
           ggTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGGGGTCTCTTTCTCCCTGGCATTCAACTGGGGCCgggg                                               >  2:170291/1‑89 (MQ=255)
              cTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGGGGTCTCTTTTTCACTGGCATTTAACTGGCGCAgggggat                                            >  2:225036/1‑86 (MQ=255)
              cTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGGGGTCTCTTTCTCACTGGCATTCAACTGGCGCAgggggat                                            >  2:113137/1‑86 (MQ=255)
              cTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGGGATCTCTTTCTCACTGGCATTCAACTGGCGCAgggggat                                            >  2:192074/1‑86 (MQ=255)
                          gATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGGGGTCTCTTTTTCACTGGCATTTAACTGGCGCCGGGCTATCTCGGGCGcccc                                >  2:160477/1‑90 (MQ=255)
                                  gatgCATCCGGTGCCGCCGGGTGTGGCGGGTGATCTCTATCTCACTGGCATTCAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGAc                        <  1:394955/90‑1 (MQ=255)
                                          cGGTGCCGCCGGGTGTGGCGGGTGATCTCTATCTCACTGGCATTCAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGcc                <  1:192074/90‑1 (MQ=255)
                                                        gtgGCGGGTGATCTCTATCTCACTGGCATTCAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGAt  <  2:275148/90‑1 (MQ=255)
                                                        gtgGCGGGTGATCTCTATCTCACTGGCATTCAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGAt  <  2:533631/90‑1 (MQ=255)
                                                                |                                                                                 
TATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGTGATCTCTATCTCACTGGCATTCAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGAT  >  NZ_CP009273/611948‑612093

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑