| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,152,727 | 0 | T | G | 60.0% | 5.8 / 13.5 | 15 | G165G (GGT→GGG) | ycfH | metal‑dependent hydrolase |
| Reads supporting (aligned to +/- strand): ref base T (1/5); new base G (9/0); total (10/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.00e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.74e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGT > NZ_CP009273/1152657‑1152816 | tGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTaa < 2:500094/73‑1 (MQ=255)tGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGaa > 1:500094/1‑73 (MQ=255) cgcgAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGGAAATAATGGATTTCggggtt > 1:270301/1‑86 (MQ=255) caggCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGa < 1:366594/88‑1 (MQ=255) ggCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGAcc < 2:217967/90‑1 (MQ=255) gTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGGAAATAATTGGTCTCGGGTTTTTTATTTCCTTTTTCTGCATTGTGGCCTTc > 1:436257/1‑90 (MQ=255) acacTGTTTTACAGAGGACAGAGAAACGGCGGGGAAATTACTTGATCTCGGGTTTTATATCTCCTTTTTCTGCATTTTGACCTTCCGTaa > 2:606998/1‑90 (MQ=255) cTGTTTTACAGAGGACAGAGAAACGGCGGGGAAAATAATGGATCTCGGGTTTTTTATCTCCTTTTTCGGCATTTTGAACTTTCGTTATgg > 1:84928/1‑89 (MQ=255) gTTTTACAGAGGACAGAGAAACGGCGGGGAAATTAATGGATCTCCGGTTTTAAATCTCCTTTTTCCGCATTTTGACCCTCCCTAATTCgg > 2:551292/1‑90 (MQ=255) gTTTTACAGAGGACAGAGAAACGGCGGGGAAAATAATTGGAATCGGGGTTTTTATCTCCTTTTTTCGCAATGTGACCTTTCCGAATGCgg > 1:521489/1‑90 (MQ=255) gTTTTACAGAGGACAGAGAAACGGCGGGGAAAATAATTGATCTCGGGTTTTAAATCTCCTTTTTCCGCATTGTGACCTTCCCTAAAGCgg > 2:447484/1‑90 (MQ=255) ttttACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGa < 1:513583/90‑1 (MQ=255) cAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAAc < 2:384614/90‑1 (MQ=255) ggACAGAGAAACGGCGGGGAAAATAATGGATCTCGGGTTTTTCATCTCCTTTTTCGGCATTTTGACCTTCCGGAATGCGGGGGAACTtcg > 2:395947/1‑90 (MQ=255) gagaAACGGCGGGTAAAATATTGGGTCTTGGGTTTTAAATCTCCTTTTTCCGCATTTTGACCTTCCGTAAATGGGAGGAACAGCCCGAtg > 2:126830/1‑90 (MQ=255) tAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGt < 1:551292/90‑1 (MQ=255) tAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGt < 2:270301/90‑1 (MQ=255) | TGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGT > NZ_CP009273/1152657‑1152816 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |