| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,199,794 | 0 | C | T | 68.4% | 4.5 / 11.7 | 19 | intergenic (‑179/‑28) | ttdR/ttdA | DNA‑binding transcriptional activator TtdR/L(+)‑tartrate dehydratase subunit alpha |
| Reads supporting (aligned to +/- strand): ref base C (1/5); new base T (13/0); total (14/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.16e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.34e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTA > NZ_CP009273/3199706‑3199879 | gTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTcc < 1:116674/90‑1 (MQ=255) cTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGGGAAATTTTTCCGACAaaaaaaaa > 2:201251/1‑90 (MQ=255) gTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGGGAAATTTTTTTGACAaaaaaaaaaa > 1:364214/1‑90 (MQ=255) cATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTTCCGACAAAAACAAAAAATGGAGtt > 2:74525/1‑90 (MQ=255) tatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGGATTTTTTCAGACAAAAACAAAAAATGGAGGTGc > 2:467372/1‑90 (MQ=255) tatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGGGGATTTTTTCAGAAAAAAAAAAAAAATGGGGTTgg > 1:49342/1‑89 (MQ=255) gTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGGAATTTTTCC‑GAAAAAAAAAAAAAAAGGAGGTGCCCtgatga > 2:614444/1‑90 (MQ=255) gTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGGGAATTTTTTCAGAAAAAAACAAAAACTGGAGGTGCCAtgatga > 2:506597/1‑90 (MQ=255) ataAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaata < 1:176770/90‑1 (MQ=255) ataAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaata < 1:492504/90‑1 (MQ=255) acaTTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTTCCGACAAAAAAAAAAAATGGAGGTGCCATGATGAGGGGAAGGAAAAAACaaaaa > 1:23606/1‑87 (MQ=255) ttttAGTGGTAGTTTGTGGC‑GGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAg < 2:49342/90‑1 (MQ=255) tAGTGGTAGTTTGTGGC‑GGTGAATTTTTTCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGAAAAAAGCAACAGGCAGTGa > 2:217014/1‑90 (MQ=255) ggTAGTTTGTGGC‑GGGGAATTTTTTCCGACAAAAACAAAAAATGGGGTTGGCCTGATGAGCGAAAAAAAAAAACAACAGGCAGTGAATaa > 2:531962/1‑90 (MQ=255) tAGTTTGTGGC‑GGTGGATTTTTTCAAACAAAAAAAAAAAACGGGGGTGGCATGATGAGGGAAAGGAAAAAACAAAAAACAGGGAAAAAgg > 1:397400/1‑89 (MQ=255) gC‑GGTGAATTTTTTCCGACAAAAACAAAAAATGGGGGTGCCCTGGTGAGGGGAAGTAAAAAACAAAAGGCAGGGAAAAAATTGACAgaga > 1:216214/1‑90 (MQ=255) gC‑GGTGAAATTTTTCCGACAAAAAAAAAAAATGGGGGTGGCATGATGAGGGGAAGAAAAAAAAAACAAGCAGGGAATAAGTTGACAgaga > 1:618741/1‑90 (MQ=255) ggTGAATTTTTTCAGACAAAAACAAAAACTGGGGGTGCCATGATGGGCGAAAGTAATAAGCAACAAGCAATGAATAAATTGACAgagaat > 1:333505/1‑88 (MQ=255) ggTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGAtt < 1:420208/90‑1 (MQ=255) ttttCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTa < 2:352352/90‑1 (MQ=255) | GTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTA > NZ_CP009273/3199706‑3199879 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |