| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,401,190 | 0 | C | G | 54.2% | 21.0 / 35.1 | 24 | G75G (GGC→GGG) | panF | sodium/pantothenate symporter |
| Reads supporting (aligned to +/- strand): ref base C (8/3); new base G (13/0); total (21/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.15e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.28e-05 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCTGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCT > NZ_CP009273/3401106‑3401278 | gCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGGCCGGGGGTGCTTTTTAATAAGGGggggggtgggg > 1:209859/1‑89 (MQ=255) ggCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGGCCGGGGCTGCTTTTAAAAAAGGGGTGGGGTGGGGAtt > 1:539760/1‑90 (MQ=255) ggCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTTTAAAAACGGGCTGGGGTGGGGAtt > 1:192584/1‑90 (MQ=255) ggCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCGGCTTTTAAAAAAGGGCTGGGGTGGGGAtt > 2:618632/1‑90 (MQ=255) ggCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGACCAGGAGCTGCTTATAAATACGGGCTGGGCTGGGTAtt > 2:531601/1‑90 (MQ=255) ccTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGGGCTGCTTTTAAAAAAGGGCTGGGGTGGGGGTTTTTGGCGGTGATTTTGCTTc > 1:32270/1‑90 (MQ=255) tatCAGTGCCAGTTCGTTTATCGGCGGGCCCGGGGGTGCTTTTAAAAAAGGGGTGGGGTGGGGGTTGTTGGGGGTGGTTTAGCCTCCTGc > 1:159663/1‑90 (MQ=255) aGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCTGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTc < 2:256626/90‑1 (MQ=255) gTGCCAGTTCGTTTATCGGCGGGGCCGGGGGTGCTTTTAAATAAGGGGTGGGGTGGGGGTTTGTGGGGGTGATTTAGCTTCCTGCCGTCt > 1:70760/1‑90 (MQ=255) gTGCCAGTTCGTTTATCGGCGGGCCCGGGGCTGCTTTTAAAAAAGGGGTGGGGTGGGGGTTGCTGGCGGGGATTTAGCTTTCTGCAGGCt > 1:114848/1‑90 (MQ=255) gTGCCAGTTCGTTTATCGGCGGGCCCGGAGCTGCTTATAAATAAGGGGTGGGGTGGGGGTTTCTGGGGGTGGTTTAGCTTCCTGCAGGCt > 1:38232/1‑90 (MQ=255) gTGCCAGTTCGTTTATCGGCGGGCCAGGGGCTGCTTTTAAATAAGGGGTGGGGTGGGGATTTCTGGGGGTGATTTAGCTTCCTTCAGTCt > 1:190351/1‑90 (MQ=255) gTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCTGGGGATTGCTGGCGATGATTCAGCTTTCTGCAGTCt > 2:126853/1‑90 (MQ=255) gTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAAAAAGGGGTGGGGTGGGGATTTCTGGCGGTGATTCAGCTTCCTCCAGGCt > 1:135637/1‑90 (MQ=255) gCCAGTTCGTTTATCGGCGGGCCAGGGGCTGCTTTTAAATACGGGCTGGGGTGGGGATTGCTGGCGGTGATTTAGCTTCCTGCAGTCTgg > 2:66605/1‑90 (MQ=255) gTTTATCGGCGGGGCCGGAGCTGCTTATAAAAACGGGGGGGGGGGGGGGTTTTTGGCGGGGATTTAGCTTCCTGCAGTCTGGCttttccc > 1:355947/1‑86 (MQ=255) gTTTATCGGCGGGCCAGGAGGTGCTTTTAAATAAGGGGTGGGGTGGGGATTTCTGGGGGTGATTTAGCTTTCTTCAGGCTGGGtttttct > 1:146882/1‑86 (MQ=255) tttATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCTGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTc < 2:86944/90‑1 (MQ=255) tgctTATAAATACGGGCTGGGCTGGGTATTGCTGGCGATGATTCGGCTTCCTGCATTCTGGCTTTCACTCGGGATTCTCGGCAAGAAGtg > 1:602300/1‑89 (MQ=255) tgctTATAAATACGGGCTGGGCTGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGtt > 2:141514/1‑90 (MQ=255) tgctTATAAATACGGGCTGGGCTGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGtt > 2:54899/1‑90 (MQ=255) gctTATAAATACGGGCTGGGCTGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGttt > 1:285018/1‑90 (MQ=255) aCGGGCTGGGCTGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTg > 2:143620/1‑90 (MQ=255) aCGGGCTGGGCTGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTg > 1:41955/1‑90 (MQ=255) cGGGCTGGGCTGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTgc < 2:291210/90‑1 (MQ=255) ggctgggctgGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTgcgc > 2:323834/1‑90 (MQ=255) ggctgggctgGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTgcgc < 1:54899/90‑1 (MQ=255) gggctgggTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCttt < 2:208307/47‑1 (MQ=255) gggctgggTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCttt > 1:208307/1‑47 (MQ=255) gctgggTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCt < 2:568262/90‑1 (MQ=255) | GCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCTGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCGCT > NZ_CP009273/3401106‑3401278 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |