Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273263,4240AC53.8% 5.8 / 11.1 14V98G (GTG→GGG) yafZDUF932 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (4/2);  new base C (0/7);  total (4/10)
Fisher's exact test for biased strand distribution p-value = 2.10e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.59e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

ACCGCACGAAATAGTCCCGGCAACATCTGATACGAACTGGTTCCATCGTGAGAGTTGAGTAGAATAATTTCCGGCACCTGTTTACCGGTGATCTGCCCTTCCCGCCGCAGACGCAGCATATGCTTTGTATGTTCACGACGACCCGGGTCACGC  >  NZ_CP009273/263349‑263501
                                                                           |                                                                             
aCCGCACGAAATAGTCCCGGCAACATCTGATACGAACTGGTTCCATCGTGAGAGTTGAGTAGAATAATTTCCGGCACCTGTTTACCGGTg                                                                 >  2:278888/1‑90 (MQ=255)
   gCACGAAATAGTCCCGGCAACATCTGATACGAACTGGTTCCATCGTGAGAGTTGAGTAGAATAATTTCCGGCACCTGTTTACCGGTGATc                                                              <  2:311981/90‑1 (MQ=255)
                  ggCAACATCTGATACGAACTGGTTCCATCGTGAGAGTTGAGTAGAATAATTTCCGGCACCTGTTTACCGGTGATCTGCCCTTCCcgccgc                                               >  1:106818/1‑90 (MQ=255)
                       cATCTAATACAAACTGTTTCCATGGGGAGAGTTGAGTAGAAAATTTTCCGGCCCCTGTTTCCGGGTGATCTGCCCTTCCCGCCGCAGACg                                          <  1:112131/90‑1 (MQ=255)
                       agtCTGATACGAACTGGTTCCTTCGTGAGAGTTGAGAAAAATATTTCCCGGCCCTTTTTTCCCGGTGATCTGCCCTCCCCGCCGCAGACg                                          <  1:120064/88‑1 (MQ=255)
                       aaTCTGATACGAACTGGTTCCATCGTGAGAGTTGAGTAGAATATTTTCGGGCTCCTGTTTACCGGTGATCTGCCCTTCCCGCCGCAGACg                                          <  2:537567/89‑1 (MQ=25)
                            gATACGAATTGTTTCCATCGTGAGAGTTGAGTAAAATATTTCCCGGCCCCTTTTTCCCGGTGTTCTCCCCTTCCCGCCGCAGACgcagca                                     <  1:278888/90‑1 (MQ=255)
                               acaaaCTGGTTCCATGGGGAGAGTGGAGTAAAATAATTTCCGGCACCTTTTTACCGGTGATCTGCCCTTCCCGCCGCAGACGCAGCatat                                  <  2:500667/87‑1 (MQ=255)
                               aCGACCGGGTTCCGTGGTGGGATTTGAGTAAAATATTTTCCGGCCTTTGTTTACCGGTGATCTGCCCTCCCCGCCGCAGACGCAGCatat                                  <  1:169205/90‑1 (MQ=255)
                                   aCTGGTTCCATCGTGAGAGTTGAGTAGAATAATTTCCGGCACCTGTTTACCGGTGATCTGCCCTTCCCGCCGCAGACGCAGCATATGcct                              >  2:120064/1‑88 (MQ=255)
                                    cTGGTCCCATCGGGAGAGTTGAGAAGAATAATTCCCGCCCCTTTTTTCCCGGTGATCTCCCCTCCCCGCCGCAGACGCAGCATATGCttt                             <  2:273793/90‑1 (MQ=255)
                                       ttcccACCGTGAGAGTTGAGAAAAAAAATTTCCGCCCCCTGTTTCCCGGTGATCTCCCCTCCCCGCCGCAGACGCAGCATATGCTTTGTa                          <  2:106818/87‑1 (MQ=255)
                                            aTCGTGAGAGTTGAGTAGAATAATTTCCGGCACCTGTTTACCGGTGATCTGCCCTTCCCGCCGCAGACGCAGCATATGCTTTGTATGTTc                     >  1:173841/1‑90 (MQ=255)
                                                               ataatTTCCGGCCCCTTTTTCCCGGTGATCCCCCCTTCCCGCCGCAGACGCAGCATATGCTTTGTATGTTCACGACGACCCGGGTcacgc  <  1:209220/90‑1 (MQ=255)
                                                                           |                                                                             
ACCGCACGAAATAGTCCCGGCAACATCTGATACGAACTGGTTCCATCGTGAGAGTTGAGTAGAATAATTTCCGGCACCTGTTTACCGGTGATCTGCCCTTCCCGCCGCAGACGCAGCATATGCTTTGTATGTTCACGACGACCCGGGTCACGC  >  NZ_CP009273/263349‑263501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑