| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 799,325 | 0 | G | T | 60.0% | 2.8 / 17.5 | 15 | V123L (GTG→TTG) | ybhJ | hydratase |
| Reads supporting (aligned to +/- strand): ref base G (2/4); new base T (9/0); total (11/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.10e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.74e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCTCCGCATATTGCGGTCATCCATCAATATATGCGTGAGATGATGGCAGGCGGCGGCAAAATGATCCTCGGGT > NZ_CP009273/799241‑799400 | tcactcTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCt < 2:522520/90‑1 (MQ=255) tctGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGGATTTTTTTGGCTcccc > 1:313961/1‑88 (MQ=255) cGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCTCCGCATATTGc < 1:901783/90‑1 (MQ=255) gTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGGATTTTTTTGCCTCCGCATATTTCg > 1:87557/1‑90 (MQ=255) cTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCTCCGCATATTGCGGTcatccatc < 2:270221/90‑1 (MQ=255) cGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTTTGCCTCCCCCTATTTCGGGCATCCCTCAatatat > 1:312775/1‑90 (MQ=255) tgatgaCCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTTTGCCTCCCCATATTTCGGGCATCCATCAATATATGCg > 1:196699/1‑90 (MQ=255) tgatgaCCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCTCCg > 1:89490/1‑61 (MQ=255) tgatgaCCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCTCCg < 2:89490/61‑1 (MQ=255) atgaCCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTTTGCCTCCGCATATTTCGGGCATCCATCAATATATGCGTg > 1:295400/1‑90 (MQ=255) atgaCCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGGATTTTTTTGCCTCCCCCTATTTCGGGCATCCCTTAAAATATGCggg > 1:826552/1‑88 (MQ=255) cATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGGGATTTTTTGGCCCCCCATATTGGGGTTATCCCTTAATATATTCGTGAggtg > 1:348625/1‑90 (MQ=255) ggcCCAGCGTTATGGCGGCATTTTTTTGCCTCCGCAAATTGCGGGCATCCATCAATATATTGGGGAGATGATTGCAGGGGGGGGGaaaaa > 1:448896/1‑89 (MQ=255) cAGCGTTATGGCGGTATTTTTGTGCCTCCGCATATTGCGGTCATCCATCAATATATGCGTGGGATGATGGCAGGCGGCGGGAAAAAGATc > 1:242009/1‑90 (MQ=255) aTGGCGGTATTTTTTTGCCTCCGCATATTTCGGGCATCCATCAATATATGCGGGGGATGATGGGCGGGGGGGGGAAAAAGAATCTCgggg > 2:452885/1‑89 (MQ=255) | TCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCTCCGCATATTGCGGTCATCCATCAATATATGCGTGAGATGATGGCAGGCGGCGGCAAAATGATCCTCGGGT > NZ_CP009273/799241‑799400 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |