| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,213,865 | 0 | G | T | 60.0% | 2.2 / 18.0 | 15 | S361I (AGT→ATT) | pdeG | cyclic diguanylate phosphodiesterase |
| Reads supporting (aligned to +/- strand): ref base G (5/1); new base T (0/9); total (5/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.00e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.74e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTGGAATTAATTTTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCC‑GTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCGGTT > NZ_CP009273/1213793‑1213949 | ttGGAATTAATTTTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCC‑GTGACAGTTTTACCCGCCGCGAtt > 1:318407/1‑90 (MQ=255)ttGGAATTAATTTTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCC‑GTGACAGTTTTACCCGCCGCGAtt > 1:811324/1‑90 (MQ=255) atttttAGCGCTTCGCATATTTTTTCGCCGACGTTTGTCGACGAGTTTTTAAATTTCC‑GTGACATTTTTACCCGCCGCGATTTAAACCtt < 1:920/88‑1 (MQ=255) ttCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCC‑GTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGt > 1:51183/1‑90 (MQ=255) aatatTTTTTCGCCGACGTTTGTCGCGGGGTTTTAAATTTTCC‑GTGACATTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCAcc < 1:515912/89‑1 (MQ=255) cGCCGACTTTTGTCGACGAGTTTTTAAATTTCC‑GTGACATTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGc < 1:718877/90‑1 (MQ=255) cGCCGACGTTTGTCGACGAGTTTTTAAATTTCC‑GTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGc < 1:297350/90‑1 (MQ=255) gcttttGCCGAGGAGT‑TTTAAATTTCCGGTGACATTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTg < 1:904348/87‑1 (MQ=255) gTCGACGAGTGTTTAAATTTCC‑GTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGtt > 1:494363/1‑90 (MQ=255) ccgaGTTTTTAATTTCCC‑GTGACATTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTtgatg < 1:122240/89‑1 (MQ=255) ttCC‑GTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAg > 1:436463/1‑90 (MQ=255) c‑GTGACTTTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCgg < 2:372929/90‑1 (MQ=255) c‑GTGACATTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCgg < 1:316264/90‑1 (MQ=255) gTGACATTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCGGt < 2:292222/90‑1 (MQ=255) tGACATTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCGGtt < 2:524471/90‑1 (MQ=255) | TTGGAATTAATTTTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCC‑GTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCGGTT > NZ_CP009273/1213793‑1213949 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |