| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,141,214 | 0 | T | G | 53.8% | 25.2 / 37.1 | 26 | G20G (GGT→GGG) | yegD | molecular chaperone |
| Reads supporting (aligned to +/- strand): ref base T (2/10); new base G (14/0); total (16/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.24e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.45e-05 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCGGCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTGACGGTAAACCGCA‑TTTGCTAAAAATGGAAAACGACAGCACGCTGCTGCCTTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCGAATG > NZ_CP009273/2141136‑2141300 | ccGGCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTGACGGGAAAACGCA‑ttt > 1:79064/1‑90 (MQ=255)ccGGCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTGACGGGAAAACCCA‑ttt > 1:191244/1‑90 (MQ=255) tCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGGGGCGGGAAAACGCA‑TTTTCTAAAAAAGGaaaaag > 1:972432/1‑88 (MQ=255) tttGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTGACGGTAAACCGCA‑TTTGCTAAAAATGGAAAACGACAGCACGctgctg < 1:835855/90‑1 (MQ=255) tttGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTGACGGTAAACCGCA‑TTTGCTAAAAATGGAAAACGACAGCACGctgctg < 1:768416/90‑1 (MQ=255) tttGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTGACGGTAAACCGCA‑TTTGCTAAAAATGGAAAACGACAGCACGctgctg < 1:603318/90‑1 (MQ=255) tGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTGACGGTAAACCGCA‑TTTGCTAAAAATGGAAAACGACAGCACGCTGCTGcc < 1:192291/90‑1 (MQ=255) aCGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTGGCGGGAAAACGCC‑TTTTCTAAAAAAAGAAAAAGACAGCACGCCGCTGCCTTCaa > 2:192291/1‑90 (MQ=255) cTGTTCAGTGGCGGTCATGCGTGGCGGGAAAACGCC‑TTTTCTAAAAATGGGAAAAGACAGCACGCTGCTGCCTTCAATGCTTTGGGCGcc > 1:639675/1‑90 (MQ=255) cTGTTCAGTGGCGGTCATGCGTGACGGGAAACCGCA‑TTTTCTAAAAAAGGAAAAAGACAGCACGCTGCTGCCTTCAATGCTTTTCGCGcc > 1:862022/1‑90 (MQ=255) cTGTTCAGTGGCGGTCATGCGTGACGGGAAACCGCA‑TTTTCTAAAAAAGGAAAAAGACAGCACGCTGCTGCCTTCAATGCTTTGCGCGcc > 2:248922/1‑90 (MQ=255) cTGTTCAGTGGCGGTCATGCGGGACGGGAAAACCCA‑TTTTCTAAAAAAGGAAAAAGACAGCACCCCGCTGCCCTCAATGCTTTTCGCGcc > 1:212163/1‑90 (MQ=255) tGTTCAGTGGCGGTCATGCGTGACGGTAAACCGCA‑TTTGCTAAAAATGGAAAACGACAGCACGCTGCTGCCTTCAATGCTTTGCGCGCCa < 1:734061/90‑1 (MQ=255) tGTTCAGTGGCGGTCATGCGTGACGGTAAACCGCA‑TTTGCTAAAAATGGAAAACGACAGCACGCTGCTGCCTTCAATGCTTTGCGCGCCa < 2:639675/90‑1 (MQ=255) gTTCAGTGGCGGTCTTGCGTGGGGGTTAAACGCAGTTTTTTAAAAAT‑GAAAAAGAAAGCACCCTCCTGCCTTCGATTCTTTTCTCGccca > 2:858062/1‑88 (MQ=255) gTTCAGTGGCGGTCATGCGTGGCGGGAAAACGCC‑TTTTCTAAAAAAAGAAAAAGACAACACCCCGCTGCCCTCAAATCTTTTCGCGccca > 2:421092/1‑88 (MQ=255) gTTCAGTGGCGGTCATGCGTGACGGGAAACCGCA‑TTTGCTAAAAAATGAAAAAGACAGCACGCCGCTGCCTTCAATGCTTTGCGCGCCaa > 1:432613/1‑90 (MQ=255) gTTCAGTGGCGGTCATGCGTGACGGGAAACCGCA‑TTTGCTAAAAAAGGAAAAAGACAGCACGCTGCTGCCTTCAATGCTTTGCGCGCCaa > 1:228116/1‑90 (MQ=255) ggTCATGCGTGGCGGGAAAACGCC‑TTTTCTAAAAAAGGAAAAAGACAGCACGCCGGTGCCTTCAATGCTTTGCGCGGCAACGCGTGAAGc > 2:775353/1‑90 (MQ=255) ggTCATGCGTGACGGTAAACCCCC‑TTTTCTAAAAATGGAAAAAGACAACACGCCGCCGCCTTCAATGCTTTTCGCGCCAACCCGTGAAGc > 2:345199/1‑90 (MQ=255) ggTCATGCGTGACGGGAAACCGCC‑TTTGCTAAAAATGGAAAACGACAGCACGCTGCTGCCTTCAATGCTTTTCGCGCCAAAGCGTGAAGc > 1:124420/1‑90 (MQ=255) ggTCATGCGTGACGGGAAAACGCA‑TTTTCTAAAAATGGAAAAAGACAGCACGCTGCTGCCTTCAATTCTTTTCGCGCCAACGCGTGAAGc > 2:88282/1‑90 (MQ=255) aTGCGTGACGGTAAACCGCA‑TTTGCTAAAAATGGAAAACGACAGCACGCTGCTGCCTTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTa < 1:870444/90‑1 (MQ=255) aCGGTAAACCGCA‑TTTGCTAAAAATGGAAAACGACAGCACGCTGCTGCCTTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCGAAt < 1:88282/90‑1 (MQ=255) aCGGTAAACCGCA‑TTTGCTAAAAATGGAAAACGACAGCACGCTGCTGCCTTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCGAAt < 1:581800/90‑1 (MQ=255) cGGTAAACCGCA‑TTTGCTAAAAATGGAAAACGACAGCACGCTGCTGCCTTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCGAATg < 1:775353/90‑1 (MQ=255) | CCGGCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTGACGGTAAACCGCA‑TTTGCTAAAAATGGAAAACGACAGCACGCTGCTGCCTTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCGAATG > NZ_CP009273/2141136‑2141300 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |