| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,356,900 | 0 | T | C | 58.3% | 0.3 / 11.6 | 13 | G71G (GGA→GGG) | yfaY | nicotinamide mononucleotide deamidase‑related protein YfaY |
| Reads supporting (aligned to +/- strand): ref base T (3/2); new base C (0/7); total (3/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.55e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.93e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAG > NZ_CP009273/2356811‑2356974 | tCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTggtcggt < 2:141025/90‑1 (MQ=255) aTCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTccc < 1:11190/90‑1 (MQ=255) tttGAGCCGGGCTTCATGCAGCCCCAGCCCTTCCCCTTTTGCTGCCGCAGCGGCGAGTGCGCTAAAATCATCGCTGGTCGGCCCCAGccc < 2:62353/90‑1 (MQ=255) cTTCATCCACCCCCAGCCCTTCCCCTTTTGCTGCCGCGGGGGCGGGTGCCTTAAAATCACCGCTGGTCGGCCCCAGCCCGCCGTTAACGa < 2:408668/90‑1 (MQ=255) tCATCCACCCCCAGCCCTCCCCTTTTTGCTGCCGCGGGGGCGAGTGCCCTAAAATCATCGCGGGTCGGCCCCACCCCGCCGTTAACGATc < 2:137700/90‑1 (MQ=255) ggCCTTCCCTTTTTGCGGCCGCGGGGGGGGGTGCGTTAAAATCACCGCGGGTCGGCCCCACCCCGCCGTTAACGATCAGCACATCGGCGt < 2:111941/90‑1 (MQ=255) gcccTTCCCTTTTTGCTGCCGCGGGGGCGAGTGCGTTTAAATCATCGCGGGTCGGCCCCAGCCCGCCGTTAACGATCAGCACATCGGCGt < 2:202564/88‑1 (MQ=255) ccTTCCCTTTTTGCTGCCGCGGGGGGGAGTGCGTTTAAATCATCGCTGGTCGGCCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGc < 2:401679/90‑1 (MQ=255) cACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGAc > 2:432392/1‑90 (MQ=255) ttttGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCCGACTACg > 2:818262/1‑90 (MQ=255) cccAGGGGGGAGCGCGTTTAAATCACCGCTGGTAGGGCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCgaag < 1:560659/88‑1 (MQ=255) atcatcGTTGGCCGGCCCCACCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAg < 1:432392/90‑1 (MQ=255) catcGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGt < 1:244806/48‑1 (MQ=255) catcGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGt > 2:244806/1‑48 (MQ=255) | TCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAG > NZ_CP009273/2356811‑2356974 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |