| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,371,314 | 0 | G | A | 50.0% | 19.3 / 10.4 | 26 | F475F (TTC→TTT) | menD | 2‑succinyl‑5‑enolpyruvyl‑6‑hydroxy‑3‑ cyclohexene‑1‑carboxylic‑acid synthase |
| Reads supporting (aligned to +/- strand): ref base G (9/4); new base A (0/13); total (9/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.58e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGA > NZ_CP009273/2371239‑2371393 | ggcgTGCTCAAAGTGGACGTTTGGCGGCATCAGAAAGAAACCCTCCCGCTCGTTTTGCGGCGTTGGCAACAGGGAAAAAATTTGCccgcc < 2:644130/90‑1 (MQ=255)ggcgTGCTAAAAAGGGACGTTTTGGGGCATCAGATAAAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCccgcc < 1:413633/90‑1 (MQ=255) cgTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCgt < 1:755051/90‑1 (MQ=255) gTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCgtt > 1:645128/1‑90 (MQ=255) gCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCgttgt > 2:937941/1‑90 (MQ=255) gCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCgttgt > 2:823028/1‑90 (MQ=255) gCTAAAAATGGCCGTTTTGCGGCATCAAATAAAACCCCCCACGCTCGTTTTGCGGCGTTGGCAACAGGGAAAAATTTTGCCCGCCgttgt < 2:639742/90‑1 (MQ=255) aaaaaTGGGCGTTTGGCGACAAAAAAAAAAACCCCCCCCGCTCGTTTGGCGGCGTTGGAAACAGCGAAAAATTTTGCCCGCCgttgttgt < 2:731109/89‑1 (MQ=255) gACGTTTTGCGGCATAAGAAAAAACCCCCCCCGCTCGTTTTGCGGCGTTGGCAACAGCGAAAAAATTTGCCCGCCGTTGTTGTTcaccac < 2:253393/90‑1 (MQ=255) gACGTTTGGCGGCATCAGAAAAAACCGCCCCCGCTCGCTTTGCGGCGTTGGCAACAGCGAAAAAATTTGCCCGCCGTTGTTGTTcaccac < 1:564062/90‑1 (MQ=255) aCGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTcacca > 2:232896/1‑88 (MQ=255) aCGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTcacca < 1:232896/88‑1 (MQ=255) ggCATCAGAAAAAACCGCTCCCCTTCGCTTGGGGGCGTTGGAAACAGCGAAAAATTTTGCCCGCCGTTGTTGTTCACCACAATTAATAcc < 2:877087/90‑1 (MQ=255) aTCAGATAGAACCGCTCCCGCTCGTTTTGGGGCGTTGCAAACAGCGAAAAAATTTCCCCGCCGTTGTTGTTCACCACAATTAATACCAgc < 2:933167/90‑1 (MQ=255) aaCGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTcaccac > 2:874390/1‑67 (MQ=255) aaCGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGaaa > 1:962048/1‑90 (MQ=255) aaCGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAAAAAATTTGCCCGCCGTTGTTGTTcaccac < 1:874390/67‑1 (MQ=255) gctTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTcacca > 2:610164/1‑53 (MQ=255) gctTTGCGGCGTTGGCAACAGCGAAAAAATTTGCCCGCCGTTGTTGTTcacca < 1:610164/53‑1 (MQ=255) gcggcgTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAg > 2:475949/1‑69 (MQ=255) gcggcgTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAg < 1:475949/69‑1 (MQ=255) gcggcgTTGGCAACAGCGAAAAAATTTCCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCa < 2:239920/90‑1 (MQ=255) cgTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAgcgc > 2:633240/1‑90 (MQ=255) gTTGGCAACAGCGAAAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGcgcg < 2:118677/90‑1 (MQ=255) gaaaCAGGGAAAAATTTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTga < 1:937941/88‑1 (MQ=255) gaaaCAGCGAAAAATTTTTCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTga < 2:962048/88‑1 (MQ=255) agaAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAAt > 2:416805/1‑65 (MQ=255) agaAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAAt < 1:416805/65‑1 (MQ=255) | GGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGA > NZ_CP009273/2371239‑2371393 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |