| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,126,661 | 0 | T | G | 60.0% | 1.4 / 16.0 | 15 | H219P (CAC→CCC) | yghS | dTMP kinase |
| Reads supporting (aligned to +/- strand): ref base T (0/6); new base G (9/0); total (9/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.00e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.47e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAAC > NZ_CP009273/3126577‑3126735 | aaaTCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTTGc > 2:739824/1‑90 (MQ=255) tcCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATcc < 1:733590/90‑1 (MQ=255) tcCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTGGCCCCTcc > 2:913519/1‑90 (MQ=255) aTCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCCGTTCa > 1:838419/1‑90 (MQ=255) aTCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTgcgc < 1:972418/90‑1 (MQ=255) aaaGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCGCCCATCCAGTTCAAGGATCTTTTCGCCAttta > 1:167295/1‑88 (MQ=255) gcgcGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATg < 2:582937/90‑1 (MQ=255) gcggcgTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGTGCCCCCCCAGGTCAAGGAGCTTTTCGTCCTTTGATGTc > 2:648571/1‑90 (MQ=255) gcggcgTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCGCCCCTCCCATTCAAAGGTCTTTTGGCCCTTGGATGtg > 2:779428/1‑89 (MQ=255) tAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCCGTTTAAGGATCTTTTCGCCATTTAATGTCAGGTGCGGGGGAACGGc > 2:580440/1‑90 (MQ=255) gCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGAttttt < 2:425290/90‑1 (MQ=255) cTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGAtttttt < 2:987384/90‑1 (MQ=255) tGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCCGTTCAAGGATCTTTTCGCCCTTTAATGTCAGTTGCGGCGGAAAGGCGGtttttttc > 2:128904/1‑88 (MQ=255) tGCAGAATTTCATCGGCGGGGGGGCGCCCATCCAGTTCAAGGGTCTTTGCGCCCTTGAATGTCAGTTGGGGCGGAAAGGCGGgttttttc > 2:250818/1‑88 (MQ=255) gAATTTCATCGGC‑GGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAG‑TGCGGCGTAACGGCGATTTTTTCCTGTAAc < 2:936227/90‑1 (MQ=255) | AAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAAC > NZ_CP009273/3126577‑3126735 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |