| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,175,957 | 0 | A | T | 59.1% | 8.3 / 25.1 | 23 | I17F (ATT→TTT) | zupT | zinc transporter ZupT |
| Reads supporting (aligned to +/- strand): ref base A (1/8); new base T (13/0); total (15/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.81e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.16e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTC‑GGGC‑AAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCAT > NZ_CP009273/3175871‑3176039 | cGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGAGCCCCGGTttttt > 2:204761/1‑89 (MQ=255) cTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGgcgc < 2:590587/90‑1 (MQ=255) tGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGcgcg < 1:553720/90‑1 (MQ=255) gAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGTAGCCGCGGTTTTTTGGGCGGTtct > 2:711569/1‑90 (MQ=255) gAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGAGCCACGGTTTTTGGGGCGGTTTTTGGCGt > 1:590535/1‑90 (MQ=255) gTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCGGGCACGTTTGTTTGCGCCTTTTTCTCCGCTCTC‑CGCC‑CAAAAcc > 2:244574/1‑90 (MQ=255) gTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTTTTGGCGCGTTTTTCGGCGTTCTC‑GGGC‑aaaaaac > 1:59105/1‑88 (MQ=255) tGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGGGCCACGGTTTTTTGCGCGCTTTTCTGCGTTTTC‑CGGCGAAAAAccc > 1:701177/1‑90 (MQ=255) aTGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTC‑GGGC‑AAAAACCCTCGAAc < 2:54966/90‑1 (MQ=255) tGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGAGCCCCGGTTTTTGGGGGGGTTTTCCGCGTTCTC‑CGGG‑AAAAAACCCCCAAcc > 2:43242/1‑90 (MQ=255) gTACCTCTCATTCTGACCATACTGGCGGGGGGAGCCACGTTTTTTGGCGCGTTTCTCGGCGTTCTC‑GGGC‑AAAAAACCTCCAACCGCTTa > 2:281237/1‑90 (MQ=255) ctctCATTCTGACCATACTGGCGGGGGCAGCCACGTTTTTTTGCGCGTTTTTCCGCGTTTTC‑CGGC‑AAAAAACCCCCAACCCCCTTCTgg > 1:761210/1‑90 (MQ=255) tctcATTCTGACCATACTGGCGGGGGGGGCCCCGGTTTTTTGCGCGGTTCTCTCCGCTCTCGGGGG‑AAAAACCCCCCAACCCCTT‑CTGGc > 2:586247/1‑90 (MQ=255) cATTCTGACCATACTGGCGGGGGGAGCCACGGTTTTTTGCGCGGTTTTCCGCGGTCTC‑CGGC‑AAAAAACCCCCAACCCCTTACTGGCggt > 2:413402/1‑88 (MQ=255) tGACCATACTGGCGGGGGCAGCCACGTTTATTTGGGCGTTTTTTCGCGTTCTC‑GGGC‑AAAAAACCCCGAACCCCTTTCTGGCGGTTTTGc > 1:596059/1‑90 (MQ=255) gACCATACTGGCGGGGGCAGCCCCGTTTTTTTGGGCGGTTTTCGGCGTTTTC‑GGGC‑AAAAAACCCCCAACCCCTTTCTGGGGGTTTTGCt > 1:955512/1‑90 (MQ=255) gACCATACTGGCGGGGGCAGCCACGTTTTTTTGGGCGTTTTTCGGCGTTCTC‑GGGC‑AAAAAACCTCGAACCGCTTTCTGGGGTTTTTGCt > 1:366031/1‑90 (MQ=255) gACCATACTGGCGGGGGCAGCCACGTTTTTTTGCGCGTTTCTCGGCGTTTTC‑GGGC‑AAAAAACCCCGAACCGCTTTCTGGCGTTTTTGCt > 1:257943/1‑90 (MQ=255) aCCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTC‑GGGC‑AAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTa < 1:586247/90‑1 (MQ=255) tACTGGCGGGGGCGGCCACGTTTTTTTGCGCGTTTCTCTGCGTTTTC‑TGGC‑AAAAACCCCCGAACCGCCTACTGGCGGTTTTGCTAGGtt > 1:191106/1‑90 (MQ=255) cGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTC‑GGGC‑AAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTgcgg < 2:257943/90‑1 (MQ=255) ggggCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTC‑GGGC‑AAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTgcggcg < 2:191106/90‑1 (MQ=255) ggggCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTC‑GGGC‑AAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTgcggcg < 2:988624/90‑1 (MQ=255) cACGTTTATTGGCGCGTTTCTCGGCGTTCTC‑GGGC‑AAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCAt < 2:596059/90‑1 (MQ=255) | CGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTC‑GGGC‑AAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCAT > NZ_CP009273/3175871‑3176039 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |