| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,058,958 | 0 | G | A | 64.7% | ‑4.6 / 14.7 | 17 | L57L (CTG→TTG) | yihO | MFS transporter |
| Reads supporting (aligned to +/- strand): ref base G (4/2); new base A (0/11); total (4/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.30e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.24e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGA > NZ_CP009273/4058870‑4059038 | aaTTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGa < 2:124164/90‑1 (MQ=255)aaTTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGa < 1:932064/90‑1 (MQ=255) cAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCAt > 1:746361/1‑90 (MQ=255) gcgAGTCGAGAAAAAACCGGGTGAGCATATCGGAAACCGGGGAAAAAACCTCCCCCCCAAAAAAAAAAACCCCGCCATAGTAAGCAGGCa < 2:152594/90‑1 (MQ=255) gagagtcgagaAAAAATCCGGTGAGAATATGGGAAACCGCGGAAAAAACCTCCGCCACCAAAAAGAAAACCCCGCCATAGTAAGCAGGCa < 1:769376/88‑1 (MQ=255) aaaaaaaaCCGGGGGAGCATATCGGAAAAGGGGAAAAAAACCTCCCCCACAAAAAAAATAACCCCGCCATAGTAAGCAGGCATCCCTAAt < 2:62322/87‑1 (MQ=255) aaaTCGGGTAAACATATCGAAAAACGCGAAAAAAACCTCCCCCGCAAAAAAAAAAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATc < 2:528011/90‑1 (MQ=255) aaaTCCGGTGAGCATCTCGGTAACCGCGATAAAAATCTTCGCCACAAAAAAAATAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATc < 1:880251/90‑1 (MQ=255) aaaCCCGGTGAGCATATGGGAAACCGCGGAAAAAAACTTCCCCCCCAAAAAAATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATc < 1:97582/90‑1 (MQ=255) aaTCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCg > 2:429250/1‑90 (MQ=255) accgggTGAGCATATCGGAAAACGGGGAAAAAAACTCCCCCCCAAAAAAAAAAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCgg < 2:285639/86‑1 (MQ=255) ggTGAGCATACCGGTAAACGCGAAAAAAACCTCCCCCACAAAAAAAAAAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGtata < 2:108895/90‑1 (MQ=255) tatCGGAAACCGCGGAAAAAACCTTCCCCACCAAAAAAATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTga < 1:49871/90‑1 (MQ=255) cGGAAAACGCGGAAAAAACCTCCCCCACAAAAAAAAAAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTgagga < 2:409250/90‑1 (MQ=255) aaaaaaCTTCCCCACAAAAAAAATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTAcc < 1:956015/90‑1 (MQ=255) tCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCaga > 2:927123/1‑90 (MQ=255) tCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCaga > 2:99269/1‑90 (MQ=255) | AATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGA > NZ_CP009273/4058870‑4059038 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |