| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,224,171 | 0 | A | C | 48.1% | 22.8 / 11.6 | 27 | P162P (CCA→CCC) | pgi | glucose‑6‑phosphate isomerase |
| Reads supporting (aligned to +/- strand): ref base A (2/12); new base C (13/0); total (15/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.98e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCCGGTGAGTGGAAAGGTTATACCGGCAAAGCAATCACTGACGTAGTGAACATCGGGATCGGCGGTTCTGACCTCGGCCCATACATGGTGACCGAAGCTCTGCGTCCGTACAAAAACCACCTGAACATGCACTTTGTTTCTAACGTCGATGGGACTCACATCGCGGAAGT > NZ_CP009273/4224091‑4224260 | tCCGGTGAGTGGAAAGGTTATACCGGCAAAGCAATCACTGACGTAGTGAACATCGGGATCGGCGGTTCTGACCTCGGCCCATACATGGTg < 2:202579/90‑1 (MQ=255)tCCGGTGAGTGGAAAGGTTATACCGGCAAAGCAATCACTGACGTAGTGAACATCGGGATCGGCGGTTCTGACCTCGGCCCATACATGGTg < 1:762932/90‑1 (MQ=255) gAGTGGAAAGGTTATACCGGCAAAGCAATCACTGACGTAGTGAACATCGGGATCGGCGGTTCTGACCTCGGCCCATACATGGTGACCGaa < 1:695097/90‑1 (MQ=255) aGTGGAAAGGTTATACCGGCAAAGCAATCACTGACGTAGTGAACATCGGGATCGGCGGTTTTTTCCTCCGCCCCTACATGGGGGCCGAAg > 1:879553/1‑90 (MQ=255) ggAAAGGTTATACCGGCAAAGCAATCACTGACGTAGTGAACATCGGGATCGGCGGGTCTGACCCCCGCCCCCACATGGGGAACGAAGcgc > 2:794409/1‑88 (MQ=255) tataCCGGCAAAGCAATCACTGACGTAGTGAACATCGGGATCGGCGGTTCTGACCCCCGCCCCCACATGGGGGCCGAAGCTCTGCGGccc > 2:163339/1‑89 (MQ=255) tataCCGGCAAAGCAATCACTGACGTAGTGAACATCGGGATCGGCGGGTTTTACCCCCGCCCCTACAAGGGGACCCAAGCTCTTCGTCCg > 1:637550/1‑90 (MQ=255) tataCCGGCAAAGCAATCACTGACGTAGTGAACATCGGGATCGGCGGGTTTTACCCCCGCCCACACATGGTGAACCAAGGGCTGCGGCCg > 2:61982/1‑90 (MQ=255) aCCGGCAAAGCAATCACTGACGTAGTGAACATCGGGATCGGCGGTTCTGACCTCGGCCCATACATGGTGACCGAAGCTCTGCGTCCGTAc < 1:148673/90‑1 (MQ=255) aCCGGCAAAGCAATCACTGACGTAGTGAACATCGGGATCGGCGGTTCTGACCTCGGCCCATACATGGTGACCGAAGCTCTGCGTCCGTAc < 1:425952/90‑1 (MQ=255) gCAAAGCAATCACTGACGTAGTGAACATCGGGATCGGCGGTTTTTACCCCCGCCCCTACATGGGGGCCGAAGCTCTGCGTCCCTACaaaa > 1:355397/1‑90 (MQ=255) gCAAAGCAATCACTGACGTAGTGAACATCGGGATCGGCGGTTTTGTCCCCCGCCCCTACATGGTGGCCGAAGGTCTGCGGCCCGACaaaa > 2:935113/1‑90 (MQ=255) gCAAAGCAATCACTGACGTAGTGAACATCGGGATCGGCGGTTCTTACCCCCGCCCCTACATGGTGACCGAAGCTCTGCGGCCGTACaaaa > 1:603130/1‑90 (MQ=255) gCAAAGCAATCACTGACGTAGTGAACATCGGGATCGGCGGTTCTTACCCCCGCCCCTACATGGGGACCGAAACTCTTCGTCCCTACaaaa > 1:845629/1‑90 (MQ=255) aaTCACTGACGTAGTGAACATCGGGATCGGCGGTTCTGACCTCGGCCCATACATGGTGACCGAAGCTCTGCGTCCGTACAAAAACCACCt < 1:922576/90‑1 (MQ=255) aCGTAGTGAACATCGGGATCGGCGGGTCTGACCTCCGCCCCCAAATGGGGACCGAAGCGCTGCGTCCGTACAAAAAACACCTGAACAttc > 1:86267/1‑88 (MQ=255) tAGTGAACATCGGGATCGGCGGTTCTTACCCCCGCCCCTACATGGGGGCCGAAAACCTGCGTCCGGACAAAAAACACCCGAACAAGCACt > 1:805204/1‑90 (MQ=255) gTGAACATCGGGATCGGCGGTTCTTACCCCCGCCCCTACATGGGGGCCGAAGCTCTTCGTCCGTACAAAAAACACCCCAACAAGCACttt > 1:440341/1‑90 (MQ=255) gTGAACATCGGGATCGGCGGGTTTGTCCCCCGCCCCCACAAGGGGAGCGAAGCGCTGCGGCCGTTCAAAAAACACCCGAAAAAGCACctt > 2:965335/1‑90 (MQ=255) gAACATCGGGATCGGCGGTTCTTTCCCCGGCCCCTACATGGGGACCGAAGCTCTGCGGCCGGACAAAAAACACCTGAACATGCACttttt > 1:122390/1‑88 (MQ=255) tCGGGATCGGCGGTTCTGACCTCGGCCCATACATGGTGACCGAAGCTCTGCGTCCGTACAAAAACCACCTGAACATGCACTTTGTTTCTa < 2:879553/90‑1 (MQ=255) cGGGATCGGCGGTTCTGACCTCGGCCCATACATGGTGACCGAAGCTCTGCGTCCGTACAAAAACCACCTGAACATGCACTTTGTTTCTaa < 2:805204/90‑1 (MQ=255) gggATCGGCGGTTCTGACCTCGGCCCATACATGGTGACCGAAGCTCTGCGTCCGTACAAAAACCACCTGAACATGCACTTTGTTTCTAAc < 2:603130/90‑1 (MQ=255) gcggTTCTGACCTCGGCCCATACATGGTGACCGAAGCTCTGCGTCCGTACAAAAACCACCTGAACATGCACTTTGTTTCTAACGTCGATg < 2:122390/90‑1 (MQ=255) gcggTTCTGACCTCGGCCCATACATGGTGACCGAAGCTCTGCGTCCGTACAAAAACCACCTGAACATGCACTTTGTTTCTAACGTCGATg < 2:863287/90‑1 (MQ=255) cGGCCCATACATGGTGACCGAAGCTCTGCGTCCGTACAAAAACCACCTGAACATGCACTTTGTTTCTAACGTCGATGGGACTCACATcgc > 1:580999/1‑90 (MQ=255) cccATACATGGTGACCGAAGCTCTGCGTCCGTACAAAAACCACCTGAACATGCACTTTGTTTCTAACGTCGATGGGACTCACATCGCGGa < 2:392096/90‑1 (MQ=255) aTACATGGTGACCGAAGCTCTGCGTCCGTACAAAAACCACCTGAACATGCACTTTGTTTCTAACGTCGATGGGACTCACATCGCGGAAGt > 1:529146/1‑90 (MQ=255) | TCCGGTGAGTGGAAAGGTTATACCGGCAAAGCAATCACTGACGTAGTGAACATCGGGATCGGCGGTTCTGACCTCGGCCCATACATGGTGACCGAAGCTCTGCGTCCGTACAAAAACCACCTGAACATGCACTTTGTTTCTAACGTCGATGGGACTCACATCGCGGAAGT > NZ_CP009273/4224091‑4224260 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |