Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,613,723	G→C	G116A (GGC→GCC)	marR →	multiple antibiotic resistance transcriptional regulator MarR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,613,723	0	G	C	100.0%	63.3 / NA	17	G116A (GGC→GCC)	marR	multiple antibiotic resistance transcriptional regulator MarR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (10/7);  total (10/7)

CCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAA  >  NZ_CP009273/1613644‑1613798                                                                                |                                                                            ccGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGCCCAGGACCTg                                                                   >  2:333644/1‑90 (MQ=255)   gAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAAC‑‑‑GCCATCAATTAGTTGCCCAGGACCTGCACCa                                                              <  2:338894/90‑1 (MQ=255)      tGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGCCCAGGACCTGCACCa                                                              <  2:978923/90‑1 (MQ=255)                 gcgTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGCCCAGGACCTGCACCAAGAATTAACaa                                                   >  1:594554/1‑90 (MQ=255)                     aCTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCACCAATTAGTTGCCCAGGACCTGCACCAAGAATTAACaaaaaa                                               >  1:516663/1‑90 (MQ=255)                      cTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGCCCAGGACCTGCACCAAGAATTAACAAAAAAc                                              >  1:5919/1‑90 (MQ=255)                      cTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGCCCAGGACCTGCACCAAGAATTAACAAAAAAc                                              >  2:28743/1‑90 (MQ=255)                           aaaaCTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGCCCAGGACCTGCACCAAGAATTAACAAAAAACCTGAc                                         >  2:326860/1‑90 (MQ=255)                                         gcggcgcggcAATATGTGAACAATGCCATCAATTAGTTGCCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTgg                           >  1:275158/1‑90 (MQ=255)                                           ggcgcggcAATATGTGAACAATGCCATCAATTAGTTGCCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCa                         <  1:356968/90‑1 (MQ=255)                                           ggcgcggcAATATGTGAACAATGCCATCAATTAGTTGCCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCa                         <  2:228716/90‑1 (MQ=255)                                                        gtgAACAATGCCATCAATTAGTTGCCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTAtt            <  2:358786/90‑1 (MQ=255)                                                               aTGCCATCAATTAGTTGCCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAac                       >  2:739932/1‑72 (MQ=255)                                                               aTGCCATCAATTAGTTGCCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAac                       <  1:739932/72‑1 (MQ=255)                                                               aTGCCATCAATTAGTTGCCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTaa     >  1:867929/1‑90 (MQ=255)                                                               aTGCCATCAATTAGTTGCCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTaa     >  1:344987/1‑90 (MQ=255)                                                                  ccATCAATTAGTTGCCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGaa  <  1:28743/90‑1 (MQ=255)                                                                                |                                                                            CCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAA  >  NZ_CP009273/1613644‑1613798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑