| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 369,093 | 0 | T | G | 53.3% | 16.1 / 19.1 | 15 | G239G (GGT→GGG) | mhpF | acetaldehyde dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (3/4); new base G (8/0); total (11/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.56e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.72e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTG > NZ_CP009273/369012‑369180 | atTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGGTATCGCCt > 2:405099/1‑90 (MQ=255) tGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTAt < 1:338081/83‑1 (MQ=255) tGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTAt > 2:338081/1‑83 (MQ=255) tGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGGGGAGGGTTTCGTTCCGGGGTATCGCCTGa > 1:441065/1‑90 (MQ=255) tGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGGGCAGGCTTACGTACCGGGGTATCGCCTGa > 1:393996/1‑90 (MQ=255) tGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAACGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGGTATCGCCTGa > 1:4299/1‑90 (MQ=255) gAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGGTTACGTACCGGGGTATCGCCTGaa > 1:258023/1‑90 (MQ=255) cTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGc < 1:417417/90‑1 (MQ=255) caAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGttt < 2:343583/90‑1 (MQ=255) gAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCg > 2:18111/1‑90 (MQ=255) atGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGGTATCGCCTGAAAAAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCgg > 1:169836/1‑90 (MQ=255) gCTGAGGCGGTGCAGGCTTACGTACCGGGGTATCGGCTGAAACAACGCGTGCAGTTTGGAGATATCCCCCAGGATAAACCGGGCAATTTa > 1:469932/1‑90 (MQ=255) ggCTTACGTACCGGGGTATCGCCTGGAAAAGCGCGGGCAGGTTGAAGTTATCCCGCCGGATAAACCGGGCAATTTACCggggggggggga > 1:393794/1‑88 (MQ=255) gggTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACt > 2:145534/1‑90 (MQ=255) ggTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTg < 1:145534/90‑1 (MQ=255) | ATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTG > NZ_CP009273/369012‑369180 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |