| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 642,203 | 0 | T | G | 55.6% | 4.8 / 23.0 | 18 | T255P (ACC→CCC) | citG | triphosphoribosyl‑dephospho‑CoA synthase CitG |
| Reads supporting (aligned to +/- strand): ref base T (0/8); new base G (10/0); total (10/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.29e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.47e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCA‑GGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTAT > NZ_CP009273/642118‑642292 | aGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCgg > 1:53405/1‑89 (MQ=255) ggTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTTGGATGc > 2:224844/1‑90 (MQ=255) tCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGccc < 2:556466/90‑1 (MQ=255) gccgccTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTGGGGTGCCCCCcttttt > 1:453704/1‑89 (MQ=255) gagaTTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTg < 2:368211/90‑1 (MQ=255) gATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTTGGATGCCCCCCTTTTTTAATAATTTTTTGGCCTCgcgccg > 1:528493/1‑88 (MQ=255) cTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTTGGATGCCCCCCTTTTTTTATAATTTTTTTGCCCCCCGCCGTAGCCa > 1:12778/1‑90 (MQ=255) tGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTTGAATGCCCCCCTTTTTCAATAATGTTTTCGCCCCCCGCTGTAGCCCGCGCa > 2:227745/1‑90 (MQ=255) tGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGGATGCCCCCCTTTTTTAATAATATTTTTGCCCCCCGCCGTAGCCCGCGCa > 1:246978/1‑90 (MQ=255) tGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGGATGCCCCCCTTTTTTAATAAATTTTTGGCCCCCCGCCGTTGCCCGCGCa > 2:421140/1‑90 (MQ=255) cGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCA‑GGc < 1:310348/90‑1 (MQ=255) aaCTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCA‑GGccc < 1:596234/90‑1 (MQ=255) aaCTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCA‑GGccc < 1:7478/90‑1 (MQ=255) aaCTGCCGGAGATAATCGAGATCGGCGGGGGGGCGAACCTCCCCCTTTTTCTATAAACTTTTCGCCCCGCGCCGTAGCCCGCGCA‑GGccc > 2:538607/1‑90 (MQ=255) gCCGGAGATAATCGAGATCGGCGGGGGGTTGAATGCCCCCCTTTTTTAATAAATTTTTTGCCCCCCGCCGTAGCCCGCGCACGGcccccc > 1:24587/1‑90 (MQ=255) ccGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCA‑GGCCCCCCTc < 1:224844/90‑1 (MQ=255) gggggTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCA‑GGCCCCCCTCGCCACCGCGCGATGCAACGtt < 1:421140/90‑1 (MQ=255) gggggTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCA‑GGCCCCCCTCGCCACCGCGCGATGCAACGtt < 2:528493/90‑1 (MQ=255) ttCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCA‑GGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTAt < 2:390138/90‑1 (MQ=255) | AGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCA‑GGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTAT > NZ_CP009273/642118‑642292 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |