| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,175,946 | 0 | C | G | 55.6% | 13.3 / 26.6 | 18 | A13G (GCA→GGA) | zupT | zinc transporter ZupT |
| Reads supporting (aligned to +/- strand): ref base C (0/8); new base G (10/0); total (10/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.29e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.38e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGTTATTTTGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCAC‑GTTTATTGGCGCGTTTCTCGGCGTTCT‑CGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCG > NZ_CP009273/3175860‑3176028 | aGTTATTTTGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGc < 2:42906/90‑1 (MQ=255) ttATTTTGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCa < 1:150465/90‑1 (MQ=255) ttATTTTGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCa < 1:438142/90‑1 (MQ=255) cGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGAGGCAC‑GGTttttt > 1:328929/1‑89 (MQ=255) cTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGGGCCAC‑GTTTTTTTgcgg > 2:567331/1‑89 (MQ=255) cTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCAC‑GTTTATTGgcgc < 1:449917/90‑1 (MQ=255) cTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCAC‑GTTTATTGgcgc < 1:91559/90‑1 (MQ=255) aGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGAGCCCC‑GCTTTTTTGGGCGTTtcttg > 2:377230/1‑88 (MQ=255) gAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGGGGCACGGTTTTTTGGGGGGTTTTTCgcgt > 1:68054/1‑87 (MQ=255) ggAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCAC‑GTTTATTGGCGCGTTTCTCGGCGTTCT‑CGGGCaaaa < 2:48517/90‑1 (MQ=255) gAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGAGCCAC‑GCTTATTTGCGCGGTTTTCCCCGTTCT‑CCGGCaaaaa > 2:500391/1‑90 (MQ=255) gTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGGGCCCC‑GGTTTTTTGGGCGGTTTTTCGCGTTCTCCGGGCAAAAAc > 1:71686/1‑90 (MQ=255) gTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGGGCCAC‑GTTTTTTTGCGCGGTTTTCGGCGGTCT‑CGGGGgaaaaac > 1:5344/1‑88 (MQ=255) ttGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGAGCCAC‑GTTTTTTTGCGCGGTTTTCGGCGTTCT‑CGGGGAAAAAacc > 1:127068/1‑90 (MQ=255) cGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCAC‑GTTTATTGGCGCGTTTCTCGGCGTTCT‑CGGGCAAAAACCCTCGa < 2:258026/90‑1 (MQ=255) cGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCAC‑GTTTATTGGCGCGTTTCTCGGCGTTCT‑CGGGCAAAAACCCTCGa < 1:299124/90‑1 (MQ=255) ctcATTCTGACCATACTGGCGGGGGGCGCCCC‑GTTTATTTGGGCGGTTTTCTGCGTTCT‑CCGGGAAAAAACCTCCAAACGCTTACTgggg > 1:537865/1‑88 (MQ=255) gCGGGGGGAGCCAC‑GTTTTTTTGGGCGTTTTTCGGCGTTCT‑CGGGCAAAAAACCCCGAACCGCTTTCTGGGGGTTTTGCTAGGTTTTtcg > 1:45518/1‑90 (MQ=255) | AGTTATTTTGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCAC‑GTTTATTGGCGCGTTTCTCGGCGTTCT‑CGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCG > NZ_CP009273/3175860‑3176028 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |