| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,578,446 | 0 | A | C | 56.2% | 3.0 / 16.7 | 16 | Y580D (TAC→GAC) | ggt | gamma‑glutamyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (7/0); new base C (0/9); total (7/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.74e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.07e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGG‑CCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATG > NZ_CP009273/3578367‑3578519 | ttACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGG‑CCGCTAACCTTAGTACCCCGCCGtt > 2:234202/1‑90 (MQ=255) aaaaaaaaaaaaGCCTCCCTTCGGCTCCCCCTAAAAAAATCCCCCTCTCCCCA‑GAAGGGGGCCCGCTACCCTTAGTCCCCCGCCGTTaaa < 2:513289/88‑1 (MQ=255) aTAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGG‑CCGCTAACCTTAGTACCCCGCCGTTAAatcat > 1:221788/1‑90 (MQ=255) aTAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGG‑CCGCTAACCTTAGTACCCCGCCGTTAAatcat > 2:507176/1‑90 (MQ=255) ccccTCCGGCTTCCCCAAAAAAAAAACCCCCCTCCCCACAAAGGGGC‑CCGCTAACCTTAGCCCCCCGCCGTTAAATCATCCACCGAgcgc < 2:389055/88‑1 (MQ=255) ggCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGG‑CCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCgg > 1:448278/1‑90 (MQ=255) gCTTCCCCTGAAAAAATACCCCTCTCCCCACGAAGGGGG‑CCGCTACCCTTAGTCCCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGa < 2:35750/90‑1 (MQ=255) tGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGG‑CCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATgc > 1:286580/1‑90 (MQ=255) tGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGG‑CCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATgc > 2:61759/1‑90 (MQ=255) tGCCCTGAAAAAATACCCCTCTCCCCACGAAGGGGC‑CCGCTACCCTTAGTCCCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATgc < 2:96792/90‑1 (MQ=255) gCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGG‑CCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATgcg > 2:485361/1‑90 (MQ=255) cccTGAAAAAACCCCCCTCTCCCCAGGAAGGGGG‑CCGCTAACCTTAGCCCCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATgcgc < 1:215313/90‑1 (MQ=255) cccTAAAAAAATCCCCCTCTCCCCACGAAGGGGC‑CCGCTACCCTTAGTCCCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATgcgc < 2:238116/90‑1 (MQ=255) cACGAGGGGGC‑CCCCTACCCTTACCCCCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCa < 1:507176/90‑1 (MQ=255) cACGAAGAGGC‑CCGCTAACCTTAGTCCCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCa < 2:51757/90‑1 (MQ=255) gcccGCTACCTTTAGCCCCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATg < 1:8361/88‑1 (MQ=255) | TTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGG‑CCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATG > NZ_CP009273/3578367‑3578519 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |