| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 49,884 | C→A | P21Q (CCG→CAG) | folA → | type 3 dihydrofolate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 49,884 | 0 | C | A | 100.0% | 80.0 / NA | 24 | P21Q (CCG→CAG) | folA | type 3 dihydrofolate reductase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (10/14); total (10/14) | |||||||||||
CAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAA > NZ_CP009273/49798‑49970 | cAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTg > 2:165318/1‑90 (MQ=255) tttATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAAcctgc < 1:261030/90‑1 (MQ=255) cAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCTGGTTTaa > 1:13443/1‑90 (MQ=255) aGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCTGGTTTaaa > 1:204604/1‑90 (MQ=255) gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCaa < 2:213871/90‑1 (MQ=255) gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCaa < 2:204604/90‑1 (MQ=255) gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCaa < 1:330201/90‑1 (MQ=255) gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCaa < 2:139348/90‑1 (MQ=255) aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAac < 2:497217/90‑1 (MQ=255) aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAac < 2:13443/90‑1 (MQ=255) gcggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACAcc > 1:6305/1‑90 (MQ=255) cggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCt < 2:6305/90‑1 (MQ=255) gcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTta < 2:440296/90‑1 (MQ=255) tAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTtaaata < 2:220774/90‑1 (MQ=255) aGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTtaaataa < 1:2508/90‑1 (MQ=255) gcgTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTa > 1:121621/1‑90 (MQ=255) gcgTTATCGGCATGGAAAACGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTa > 1:216368/1‑90 (MQ=255) ttATCGGCATGAAAACCGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATgg < 1:165318/90‑1 (MQ=255) aggaaaaaCCCCATGCAGGGAACCCTCCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAt < 1:518478/86‑1 (MQ=255) aCGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTggg > 1:456574/1‑90 (MQ=255) cGCCATGCAGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGa > 2:558209/1‑90 (MQ=255) aTGCAGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATgg < 1:403598/70‑1 (MQ=255) aTGCAGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATgg > 2:403598/1‑70 (MQ=255) tGCAGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatcaa > 1:548265/1‑90 (MQ=255) | CAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAA > NZ_CP009273/49798‑49970 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |