Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,632,461	T→G	L487R (CTT→CGT)	pitA →	inorganic phosphate transporter PitA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,632,461	0	T	G	100.0%	151.4 / NA	40	L487R (CTT→CGT)	pitA	inorganic phosphate transporter PitA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (19/21);  total (19/21)

GGTAGATGGTGGCGGCTTACAGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGAT  >  NZ_CP009273/3632378‑3632544                                                                                    |                                                                                    ggtAGATGGTGGCGGCTTACAGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCcgg                                                                               >  1:571478/1‑90 (MQ=255) ggtAGATGGTGGCGGCTTACAGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCcgg                                                                               <  1:345834/90‑1 (MQ=255)      aTGGTGGCGGCTTACAGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGc                                                                          <  1:473372/90‑1 (MQ=255)      aTGGTGGCGGCTTACAGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGc                                                                          >  1:424212/1‑90 (MQ=255)       tggtggCGGCTTACAGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGCt                                                                         <  1:351442/90‑1 (MQ=255)        ggtggCGGCTTACAGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTg                                                                        >  1:36488/1‑90 (MQ=255)         gtggCGGCTTACAGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTGt                                                                       >  1:233491/1‑90 (MQ=255)         gtggCGGCTTACAGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTGt                                                                       >  1:552863/1‑90 (MQ=255)           ggcggcTTACAGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTAc                                                                     <  2:470264/90‑1 (MQ=255)           ggcggcTTACAGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTAc                                                                     <  2:478304/90‑1 (MQ=255)            gcggcTTACAGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACt                                                                    >  1:347990/1‑90 (MQ=255)                          aaaaCCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAg                                                      >  2:465506/1‑90 (MQ=255)                          aaaaCCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAg                                                      <  2:328516/90‑1 (MQ=255)                            aaCCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGtt                                                    <  2:181998/90‑1 (MQ=255)                            aaCCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGtt                                                    <  1:547242/90‑1 (MQ=255)                              ccGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTcc                                                  <  2:347990/90‑1 (MQ=255)                              ccGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTcc                                                  <  1:302508/90‑1 (MQ=255)                                gTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTg                                                >  1:452239/1‑90 (MQ=255)                                gTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTg                                                >  2:465850/1‑90 (MQ=255)                                gTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTg                                                >  1:407594/1‑90 (MQ=255)                                   aCCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTaa                                             <  1:402971/90‑1 (MQ=255)                                    ccAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAAt                                            <  2:144403/90‑1 (MQ=255)                                       gCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGc                                                                          >  2:260184/1‑57 (MQ=255)                                       gCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGc                                                                          <  1:260184/57‑1 (MQ=255)                                                 ggCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTATTCGTACGCACCaaa                               >  2:595513/1‑90 (MQ=255)                                                 ggCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCaaa                               >  1:370570/1‑90 (MQ=255)                                                      gggTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGc                                                                          >  2:504050/1‑42 (MQ=255)                                                      gggTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGc                                                                          <  1:504050/42‑1 (MQ=255)                                                      gggTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAg                          >  2:129433/1‑90 (MQ=255)                                                      gggTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAg                          >  2:385051/1‑90 (MQ=255)                                                       ggTGTTTACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGc                         <  2:452239/90‑1 (MQ=255)                                                           tttACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGc                         <  2:577652/86‑1 (MQ=255)                                                           tttACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGc                         >  1:577652/1‑86 (MQ=255)                                                             tACCCTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCa                   >  1:491555/1‑90 (MQ=255)                                                                ccTTCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCt                <  2:96462/90‑1 (MQ=255)                                                                  ttCCGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTgg              >  1:107842/1‑90 (MQ=255)                                                                    ccGGCTGCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGcc            <  1:549184/90‑1 (MQ=255)                                                                          gCGGTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTc      <  2:424212/90‑1 (MQ=255)                                                                            ggTACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAg    <  2:491555/90‑1 (MQ=255)                                                                              tACTGCGTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGAt  <  2:483282/90‑1 (MQ=255)                                                                                    |                                                                                    GGTAGATGGTGGCGGCTTACAGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTGTTTACCCTTCCGGCTGCGGTACTGCTTTCCGGCGGGCTGTACTGGCTCTCCTTGCAGTTCCTGTAATCGTACGCACCAAAACGAGCGGGTCAGCTGGCCCGCTTCAGAT  >  NZ_CP009273/3632378‑3632544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑