| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 517,106 | 0 | T | G | 53.8% | 8.4 / 12.1 | 13 | V412G (GTA→GGA) | ybbP | ABC transporter permease |
| Reads supporting (aligned to +/- strand): ref base T (1/5); new base G (7/0); total (8/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.66e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.70e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GATTGTCAGTGTGGTGGTTGTGCTGCTGCTCGCCGGATTAATGGGTGGCAGCATGCTGCTTTGGGCGGTGCTGGCGGGCGCGGTAGTACTGGCTTTGCTGTGCGGTGTGCTGGGCTGGATGCTGCTGAATGTACTTCGCCGCATGACGCTGAAATCGCTGCCTCTGC > NZ_CP009273/517023‑517189 | gATTGTCAGTGTGGTGGTTGTGCTGCTGCTCGCCGGATTAATGGGTGGCAGCATGCTGCTTTGGGCGGTGCTGGCGGGCGCGGTAGTACt < 1:556702/90‑1 (MQ=255) aGTGTGGTGGTTGTGCTGCTGCTCGCCGGATTAATGGGTGGCAGCATGCTGCTTTGGGCGGTGCTGGCGGGCGCGGGAGTACTGGCTTTg > 2:705274/1‑90 (MQ=255) tggtTGTGCTGCTGCTCGCCGGATTAATGGGTGGCAGCATGCTGCTTTGGGCGGTGCTGGCGGGCGCGGTAGTACTGGCTTTGCTGTGCg > 2:497992/1‑90 (MQ=255) tgtgCTGCTGCTCGCCGGATTAATGGGTGGCAGCATGCTGCTTTGGGCGGTGCTGGCGGGCGCGGGAGGACTGGCTTTGCTGTGCggggg > 2:3778/1‑87 (MQ=255) tgctgctCGCCGGATTAATGGGTGGCAGCATGCTGCTTTGGGCGGTGCTGGCGGGGGGGGGAGTAGTGGCTTTGCTGTGGggtggggtgg > 1:122156/1‑90 (MQ=255) ccGGATTAATGGGTGGCAGCATGCTGCTTTGGGCGGTGCTGGCGGGCGCGGTAGTACTGGCTTTGCTGTGCGGTGTGCTGGGCTGGAtgc < 1:497992/90‑1 (MQ=255) gATTAATGGGTGGCAGCATGCTGCTTTGGGCGGTGCTGGCGGGCGCGGTAGTACTGGCTTTGCTGTGCGGTGTGCTGGGCTGGAtgctgc < 1:339449/90‑1 (MQ=255) tcaaTGGGTGGCAGCATGCTGCTTTGGGCGGTGCTGGGGGGCGCGGTAGTAGTGGCTTTGCTGTGCGGTGTGCTGGGCTGGATGctgctg < 1:166753/88‑1 (MQ=255) gctgctTTGGGCGGTGCTGGCGGGCGCGGGAGTACTGGCTTTGCTGTGGGGGGGGGTGGGCTGGGTGGTGCTGGATGTACTTtgccgcct > 1:762392/1‑88 (MQ=255) gggCGGTGCTGGCGGGCGCGGGAGTACTGGCTTTGCTGTGCGGGGTGCTGGGCGGGGTGGTGCTGAATGTACTTCGCCGCCTGACGCTGa > 1:322128/1‑90 (MQ=255) gCTGGCGGGCGCGGTAGTACTGGCTTTGCTGTGCGGTGTGCTGGGCTGGATGCTGCTGAATGTACTTCGCCGCATGACGCTGAAATCGCt < 2:703137/90‑1 (MQ=255) ggcgCGGGAGTACTGGCTTTTCTGTGCGGGGGGCTGGGGTGGGTGCTGCTGAATGTACTTCGCCGCCTGACGCTGAAATCGCCGCCTCTg > 1:619150/1‑90 (MQ=255) gcgcGGGAGTACTGGCTTTGCTGTGCGGTGTGCTGGGCTGGATGCTGCTGAATGTACTTCGCCGCATGACGCTGAAATCGCTGCCTCTgc > 2:70074/1‑90 (MQ=255) | GATTGTCAGTGTGGTGGTTGTGCTGCTGCTCGCCGGATTAATGGGTGGCAGCATGCTGCTTTGGGCGGTGCTGGCGGGCGCGGTAGTACTGGCTTTGCTGTGCGGTGTGCTGGGCTGGATGCTGCTGAATGTACTTCGCCGCATGACGCTGAAATCGCTGCCTCTGC > NZ_CP009273/517023‑517189 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |