| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 518,782 | 0 | T | G | 60.0% | 6.0 / 12.6 | 15 | G22G (GGT→GGG) | rhsD | rhs element protein RhsD |
| Reads supporting (aligned to +/- strand): ref base T (1/5); new base G (9/0); total (10/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.00e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.74e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCG > NZ_CP009273/518695‑518869 | aaTAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCCTTTTCCAAGGttt > 2:416458/1‑89 (MQ=39) aTAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCCTTTTCCAGGGTTCg > 2:315591/1‑90 (MQ=255) aTAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCCTTGTCCCGGGGTCg > 1:77826/1‑90 (MQ=37) aTAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGGTCg > 2:496630/1‑90 (MQ=255) aaCTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGc < 1:392055/90‑1 (MQ=255) cTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAg < 1:416458/90‑1 (MQ=255) tAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTTTCCAGGGGTCGGCAgg > 2:47713/1‑90 (MQ=255) aCTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCCCTTGCCCGGGGTCCGCGGGGGTAAGaaa > 1:455131/1‑89 (MQ=25) tttATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCCTTTTCCAGGGGTCGGCAGGGGTAAGaaatt > 2:477383/1‑87 (MQ=21) gCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCCTTGTCCAGGGGTCGGCAGGGGTGAGAAATGGGCCGcccc > 1:724843/1‑89 (MQ=18) gCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGGTCGGCAGGGGTAAGAATTGGCGCGCCCa > 2:30377/1‑90 (MQ=255) gCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCAATTTCCCGGGGTCGGGAGGGGTAAAAAATGGGGGGcccc > 1:467717/1‑89 (MQ=18) gcgTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGCGTGCTc < 2:467717/90‑1 (MQ=255) gCGGTCCCATTGTCCAGGGGTCGGCAGGGGTAAGAATTTGGGCGCCCCCCCGCGGGGCGGGGTCGGGGGGTCCCGGGGGGGTGACTTCgg > 1:320938/1‑90 (MQ=255) gTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCa < 1:505636/90‑1 (MQ=255) ggTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCg < 1:47713/90‑1 (MQ=255) | AATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCG > NZ_CP009273/518695‑518869 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |