Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273822,9550TG57.1% 15.6 / 26.6 22T495P (ACC→CCC) ybhFATP‑binding cassette domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (0/9);  new base G (12/0);  total (13/9)
Fisher's exact test for biased strand distribution p-value = 3.40e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.41e-05
Rejected as consensus: Frequency below/above cutoff threshold.

CTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGC  >  NZ_CP009273/822871‑823037
                                                                                    |                                                                                  
cTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGgggggggg                                                                               >  2:89793/1‑90 (MQ=255)
              tgggtggTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCGACGCCGGaa                                                                 >  1:191712/1‑90 (MQ=255)
               gggtggTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGAACGCCGGGAg                                                                >  1:206152/1‑90 (MQ=255)
               gggtggTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGAAAGCCGGAAg                                                                >  2:411724/1‑90 (MQ=255)
                    gtgACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGGAGGCggg                                                           >  2:348069/1‑89 (MQ=255)
                     tgACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGGACGCCGGGAGGCgggt                                                          >  1:390066/1‑88 (MQ=255)
                          aTCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAAAGCCGGGAGGCGGGtcgtcg                                                     >  2:55285/1‑90 (MQ=255)
                              ccGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGAACGCCGGGAGGCGGGTCGTCGAGaa                                                 >  1:421302/1‑90 (MQ=255)
                                  gACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCCGAAGGCGGGTCGTCGGGAAACaa                                             >  2:440313/1‑89 (MQ=255)
                                   aCGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGa                                            <  1:55285/90‑1 (MQ=255)
                                    agCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGaa                                           <  2:689582/89‑1 (MQ=255)
                                         ttCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTcc                                      <  2:498320/90‑1 (MQ=255)
                                           ctctACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCgg                                    <  1:251476/90‑1 (MQ=255)
                                                ccATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGAAAGCCGGGAGGCGGGTCGTCGGGAAAAAGAAAGTCCGGGTcct                               >  1:689582/1‑88 (MQ=255)
                                                  aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGc                             <  2:414833/90‑1 (MQ=255)
                                                  aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGAACGCCGGGAGGCGGTTTGTCGAGAAAAAGAAAGTCCGGGTTATGc                             >  1:414833/1‑90 (MQ=255)
                                                  aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGAAAACGGTTTGTCGGGAAACAGAATGTCCCGTTCTTGc                             >  2:287029/1‑90 (MQ=255)
                                                  aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGCGAGGGGGGAAACCCCGGAAGGCCGTTCGTCGGGAAACAAAATTTCCGGGTCAttg                             >  2:544895/1‑88 (MQ=255)
                                                       gTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAg                        <  2:621770/90‑1 (MQ=255)
                                                               gCAGCCAAAATTCACGGCGGGGGGGGGGGGGGACGCCCGGAGTCGGTTCGTCGGGAAAAAAAAAGTCCGGGTTATGCATCAGCGAACAgg                >  1:621770/1‑90 (MQ=255)
                                                                 aGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGgcc              <  2:502575/90‑1 (MQ=255)
                                                                         ttCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGgccagcgccag      <  2:199825/90‑1 (MQ=255)
                                                                             cggcggGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGc  <  2:206152/90‑1 (MQ=255)
                                                                                    |                                                                                  
CTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGC  >  NZ_CP009273/822871‑823037

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑