Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273822,9630TG56.5% 9.8 / 30.0 23D492A (GAC→GCC) ybhFATP‑binding cassette domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (0/10);  new base G (13/0);  total (13/10)
Fisher's exact test for biased strand distribution p-value = 8.74e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.23e-05
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAA  >  NZ_CP009273/822885‑823045
                                                                              |                                                                                  
tgggtggTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCGACGCCGGaa                                                                         >  1:191712/1‑90 (MQ=255)
 gggtggTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGAACGCCGGGAg                                                                        >  1:206152/1‑90 (MQ=255)
 gggtggTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGAAAGCCGGAAg                                                                        >  2:411724/1‑90 (MQ=255)
      gtgACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGGAGGCggg                                                                   >  2:348069/1‑89 (MQ=255)
       tgACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGGACGCCGGGAGGCgggt                                                                  >  1:390066/1‑88 (MQ=255)
            aTCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAAAGCCGGGAGGCGGGtcgtcg                                                             >  2:55285/1‑90 (MQ=255)
                ccGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGAACGCCGGGAGGCGGGTCGTCGAGaa                                                         >  1:421302/1‑90 (MQ=255)
                    gACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCCGAAGGCGGGTCGTCGGGAAACaa                                                     >  2:440313/1‑89 (MQ=255)
                     aCGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGa                                                    <  1:55285/90‑1 (MQ=255)
                      agCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGaa                                                   <  2:689582/89‑1 (MQ=255)
                           ttCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTcc                                              <  2:498320/90‑1 (MQ=255)
                             ctctACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCgg                                            <  1:251476/90‑1 (MQ=255)
                                  ccATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGAAAGCCGGGAGGCGGGTCGTCGGGAAAAAGAAAGTCCGGGTcct                                       >  1:689582/1‑88 (MQ=255)
                                    aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGc                                     <  2:414833/90‑1 (MQ=255)
                                    aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGAACGCCGGGAGGCGGTTTGTCGAGAAAAAGAAAGTCCGGGTTATGc                                     >  1:414833/1‑90 (MQ=255)
                                    aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGAAAACGGTTTGTCGGGAAACAGAATGTCCCGTTCTTGc                                     >  2:287029/1‑90 (MQ=255)
                                    aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGCGAGGGGGGAAACCCCGGAAGGCCGTTCGTCGGGAAACAAAATTTCCGGGTCAttg                                     >  2:544895/1‑88 (MQ=255)
                                         gTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAg                                <  2:621770/90‑1 (MQ=255)
                                                 gCAGCCAAAATTCACGGCGGGGGGGGGGGGGGACGCCCGGAGTCGGTTCGTCGGGAAAAAAAAAGTCCGGGTTATGCATCAGCGAACAgg                        >  1:621770/1‑90 (MQ=255)
                                                   aGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGgcc                      <  2:502575/90‑1 (MQ=255)
                                                           ttCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGgccagcgccag              <  2:199825/90‑1 (MQ=255)
                                                               cggcggGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGc          <  2:206152/90‑1 (MQ=255)
                                                                       gAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTaaa  <  1:440313/90‑1 (MQ=255)
                                                                              |                                                                                  
TGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAA  >  NZ_CP009273/822885‑823045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑