| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 832,212 | 0 | T | G | 60.0% | 9.4 / 20.4 | 20 | F136V (TTT→GTT) | hcxB | hydroxycarboxylate dehydrogenase HcXB |
| Reads supporting (aligned to +/- strand): ref base T (1/7); new base G (12/0); total (13/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.03e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.44e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTC > NZ_CP009273/832125‑832301 | cAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGttt < 2:456112/90‑1 (MQ=255)cAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGttt < 1:323841/90‑1 (MQ=255) cACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGtc < 1:365317/90‑1 (MQ=255) gccgTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGGTTGTCTCTATCCCCttt > 1:580932/1‑90 (MQ=255) cgTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGGTTGTCTCTTTCCACttttt > 1:515860/1‑88 (MQ=255) tACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTgg < 1:392486/90‑1 (MQ=255) cATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTc < 2:744300/90‑1 (MQ=255) tCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGGTTTTCTCTTTCCACTTTTTTTGCGTGGGCGGGAtt > 2:517964/1‑90 (MQ=255) cGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGGTTGTGTCTATCCACTTTGTTAGCGTGGGCGGGATTGCCAAGGTCGGGccc > 1:330471/1‑89 (MQ=255) gTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGGTTGTCTCTTTTCCCTTTTTTTGGGGGGGCGGGATTCCGAAGGTCGCGCCGt > 1:752294/1‑90 (MQ=255) tATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGGTTTTCTCTATCCACTTTTTTAGGGGGGGCGGGATTTCAATGGGGGCGCCggt > 1:280082/1‑88 (MQ=255) tATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGGTTGTCTCTTTCCCCCTTGTTTGAGGGGGTGGGATTCCAAATGTGGCGCCggt > 1:285630/1‑88 (MQ=255) gggCGGAGCAGTGTGCAGCGGCGGGGGTTGTCTCTATCCACTTTTTTAGCGGGGTCGGGATTCCAATGGTCGCGCCGTTCCCCGGTcgcg > 2:272257/1‑90 (MQ=255) ggAGCAGTGTGCAGCGGCGGGGGTTGTCTCTTTCCACTTTTTTTGCGTGGGCGGGATTCCAAAAGGGGCGGCGTTTCCCCGTcgcggccg > 1:203285/1‑86 (MQ=255) aGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGcc < 2:381443/90‑1 (MQ=255) gtgtGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGGCGGGATTCCAATGGTCGCGCCGTTCCACGGGCGCGACAGCCGcct > 2:624918/1‑88 (MQ=255) gtgtGCAGCGGCGGGGGTTTTTTCTATCCACTTTTTTAGCGGGGGCGGGATTCCAATGGGCGCGCCGTTCCCCGGTCGCGACAGCCGCtt > 1:513830/1‑90 (MQ=255) gtgtGCAGCGGCGGGGGTTGTCTCTATCCACTTTGTTTGCGTGGTCGGGATTCCAATGGTCGCGCCGTTCCACGGGCGCGACAGCCGCtt > 2:765208/1‑90 (MQ=255) tgtgCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCttt < 2:742630/90‑1 (MQ=255) cggcggGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCa < 1:736473/90‑1 (MQ=255) cggGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATc < 1:677444/90‑1 (MQ=255) ggggGTTTTTTCTATCCACTTTTTTTGCGGGGGCGGGATTCCAAAGGTCGGGCCGTTCCACCGTCGCGACAGCCGCCTTGGGACCCATcc > 1:507565/1‑90 (MQ=255) tttGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTc < 1:697053/90‑1 (MQ=255) | CAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTC > NZ_CP009273/832125‑832301 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |