| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 890,386 | 0 | A | C | 53.8% | 11.5 / 14.5 | 13 | intergenic (+34/‑61) | potF/potG | spermidine/putrescine ABC transporter substrate‑binding protein PotF/putrescine ABC transporter ATP‑binding subunit PotG |
| Reads supporting (aligned to +/- strand): ref base A (5/1); new base C (0/7); total (5/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.66e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.56e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAG > NZ_CP009273/890305‑890473 | gACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCgg > 1:706847/1‑90 (MQ=255) gcgcCCCCGCGGGTGGACAAAAGGGAAGAGCGGAAAATAACCCGCAGTCGTAGAGGCCGGAGGGGCGCACCACCCCCGCCGGCAATTCGc < 1:482787/90‑1 (MQ=255) gcgcACCGGGGCGAGGAAAAAGGGGAAGGGGGGAAAAAAATCCGAAGTCGTGGATGCGGGGGGGGCGCACCACACCCGCCGGCAATTCGc < 1:372646/90‑1 (MQ=255) cccccccCGCGTGGACAAAAGTGAAGGGCGGAAAATAATCCGCAGTTTAAGATGCCGGGGGGGCGCCCCACCCCCGCCGGCAATTCGCAc < 2:763405/87‑1 (MQ=255) gcgcgTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACCCCCGCCGGCAATTCGCACCATTAt < 2:127511/90‑1 (MQ=255) gcgcgTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTAt > 2:365186/1‑90 (MQ=255) caaaaGTGAAGAGCGAAAAATAACCCGCAGTCGTAGATGCGGGAGGGGCCCACCACCCCCGCCGGCAATTCGCACCATTATGGTGCGCtt < 2:536986/88‑1 (MQ=255) aaGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACAt > 1:556609/1‑90 (MQ=255) gagCGGAAAATAATCCGCAGTGGAAGAGGCGGGGGGGGCCCCCCCCCCCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTc < 1:31241/90‑1 (MQ=255) cccGCAGTCGTAGATGCGGGGGGGGCCCCCCACCCCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGagc < 2:556609/89‑1 (MQ=255) gCAGTCGTAGATGCCGGAGGGGCGCACCACCCCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGagcagc < 2:539890/90‑1 (MQ=255) ccGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCt > 2:774732/1‑90 (MQ=255) aagcgcACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCt > 2:310536/3‑90 (MQ=255) acacCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAg > 2:427247/1‑90 (MQ=255) | GACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAG > NZ_CP009273/890305‑890473 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |