| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 937,169 | 0 | T | G | 60.0% | ‑2.6 / 20.8 | 20 | V252G (GTG→GGG) | dmsA | dimethylsulfoxide reductase subunit A |
| Reads supporting (aligned to +/- strand): ref base T (0/8); new base G (12/0); total (12/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.94e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.43e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCG > NZ_CP009273/937103‑937252 | aaaaCAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCAc < 2:791515/90‑1 (MQ=255) gTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGGCTTTCTTTCTTGAAAAGGCACGCCCGa > 2:626363/1‑90 (MQ=255) ggTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGGGTTACTATTTTTAACAAACACGCCAAAAAACTaa > 1:270459/1‑90 (MQ=255) gTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACCTAATATCTTGAACAGGCACGCCAGGAATCTAAt > 2:771163/1‑90 (MQ=255) aGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTGAACAGGCACGCCAGAAATCTAAttc > 2:315695/1‑88 (MQ=255) tGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCa < 1:599532/90‑1 (MQ=255) ttGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTTAACAGGCACGCCAGAAAACTAATGCCCGCATGa > 2:391746/1‑90 (MQ=255) ttGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGGGTTTATATTTTTAAAAAGGACGCCACAAAACTAATACCCCCATGa > 1:164241/1‑90 (MQ=255) taaCCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGatcatcat < 1:410167/90‑1 (MQ=255) aaCCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGGGTTACTATCTTTAAAAGGCACGCCAGAAAACTAATGCCCGCCTGatcatcatc > 1:330111/1‑90 (MQ=255) cTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATc < 2:669345/90‑1 (MQ=255) cTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTTCTATCTTTAAAAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATc > 2:164199/1‑90 (MQ=255) tGGCGAAACGCGAATGAGTGGCGGTGGGGGGGGTTACTATCTTTAAAAAGCACGCCCGAAAACTAATTCCCCCCTGATCATCATCGATcc > 1:54255/1‑90 (MQ=255) tGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTTCTATCTTTAAAAAGCACCCCCGAAAACTAATGCCCGCAAGATCATCatccatcc > 1:369263/1‑90 (MQ=255) tGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTTAACAGGCACGCCAGAAAACTAATTCCCCCCTGATCATCATCGATcc > 2:507587/1‑90 (MQ=255) tGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTGAACAAGCACGCCCGAAAACTAATGCCCCCATGATCATCATCGATcc > 2:429920/1‑90 (MQ=255) cGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCcgcg < 2:54255/90‑1 (MQ=255) gAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCg < 2:370731/90‑1 (MQ=255) gTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCg < 2:270459/90‑1 (MQ=255) gTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCg < 1:269768/90‑1 (MQ=255) | AAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCG > NZ_CP009273/937103‑937252 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |