| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,152,727 | 0 | T | G | 60.0% | 1.9 / 12.3 | 15 | G165G (GGT→GGG) | ycfH | metal‑dependent hydrolase |
| Reads supporting (aligned to +/- strand): ref base T (1/5); new base G (9/0); total (10/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.00e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.34e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATC‑TCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGT > NZ_CP009273/1152641‑1152816 | gATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTaaa < 2:490081/90‑1 (MQ=255) ttCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCg < 2:117586/90‑1 (MQ=255) cGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATC‑TCCTTTTCCg < 1:590862/90‑1 (MQ=255) cGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGAAAATATTGGGTCTCGGGTTTTTTATC‑TCCtttttcc > 2:9716/1‑87 (MQ=255) ggATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATC‑TCCTTTTCCgg < 1:784006/90‑1 (MQ=255) ttGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAAATATTTTACCTGGGGTTTTATATC‑CCCTTTTTCggtta > 2:6694/1‑87 (MQ=255) acacTGTTTTACAGAGGACAGAGAAACGGCGGGGAAAATAATGGATCTCGGGTTTTTAATC‑TCCTTTTTCCGCCTTGTGGCCTTCCGTaa > 2:306157/1‑90 (MQ=255) cTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATC‑TCCTTTTCCGGCATTGTGACCTTCCGTAATGc < 2:215199/90‑1 (MQ=255) gTTTTACAGAGGACAGAGAAACGGCGGGGGAAATAATTGATTTTGGGTTTTTTATC‑TTCTTTTTCCGCCTTGTGTCCCTCCCTTATGCgg > 1:777976/1‑90 (MQ=255) gTTTTACAGAGGACAGAGAAACGGCGGGGAAATTACTGGATCTCGGGTTTTATATC‑TCCTTTTTCGGCATTGTGGCCTTCCCTAATGAgg > 2:35907/1‑90 (MQ=255) gTTTTACAGAGGACAGAGAAACGGCGGGGAAATTAATTGGTTTCGGATTTTTAATC‑TCCTTTTTCGGCATTTTGACCCTCCCTAATGCgg > 2:497601/1‑90 (MQ=255) ggACAGAGAAACGGCGGGGGAAATAATGGAGTTCGGGTTTTTAATC‑TCCTTTTTCGGCATTGTTAGCCTCCGTAATGCGGGGGAACTtcg > 1:307242/1‑90 (MQ=255) ggACAGAGAAACGGCGGGGAAAATACTGGATCTCGGGGTTTATATC‑TCCTTTTTCGGCCTTTTGGGCTTCCTTAATGCGGAGCAACTgcg > 2:208918/1‑90 (MQ=255) ggACAGAGAAACGGCGGGGAAAATAATTGATCTCCGGTTTTAAAACTTCCTTTTCCGCCA‑TGTGACCTTCCGTAATGCGGAGGAACcgcg > 1:19332/1‑90 (MQ=255) gagaAACGGCGGGGAAAATACTGGATCTCGGATTTTAAATC‑TCCTTTTTCCGCATTTTTGCCTTCCGTAATTCGGGGCAAATGCGCGGtg > 2:523535/1‑90 (MQ=255) tAAATTACTGGATCTCGGATTTTACATC‑TCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGt < 1:678291/90‑1 (MQ=255) tAAATTACTGGATCTCGGATTTTACATC‑TCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGt < 1:6694/90‑1 (MQ=255) tAAATTACTGGATCTCGGATTTTACATC‑TCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGt < 2:786479/90‑1 (MQ=255) | GATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATC‑TCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGT > NZ_CP009273/1152641‑1152816 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |