| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,500,285 | 0 | A | C | 60.0% | 7.8 / 15.7 | 15 | V18G (GTA→GGA) | ydcO | BenE family transporter YdcO |
| Reads supporting (aligned to +/- strand): ref base A (5/1); new base C (0/9); total (5/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.00e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.47e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACC > NZ_CP009273/1500197‑1500369 | cATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATAc < 1:715060/90‑1 (MQ=255) ccGGCAACAACCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCAAATTAATCCCGCCAAAACCCCCGCCAGTAGCGTGGGTgga < 2:388105/90‑1 (MQ=255) aaaaaTCGCTGCTCGCCAAATTTTGCCCGCTAAACGGGCGTAGCAAATTAATCCCGCAAAAAACCCCGCCAGTAGCGTGGGTGGAGGAAt < 2:187110/87‑1 (MQ=255) aTCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGaa > 1:203270/1‑90 (MQ=255) ttGCAAGATTATTGCCGCTAAACGGGCGTAGCAAATTAATCCCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACg < 2:295716/90‑1 (MQ=255) tggCCGCTGAACTGGGGTACCAAATTAATCCCGCAAAAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCt < 2:231018/88‑1 (MQ=255) cccGCTGACCGGGCGTAGCCAATTAATCCCGCAAAAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCg < 2:736029/89‑1 (MQ=255) gCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGc > 2:65840/1‑90 (MQ=255) gaaaCGGGCGTAGCAAATAAATCCCCCCAAAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGt < 2:114885/88‑1 (MQ=255) cgggCGTAGCAAATAAACCCCGCAAAAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTtata < 2:203270/88‑1 (MQ=255) ggCGTACCCAATAAACCCCCCCAAAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGc < 2:102416/90‑1 (MQ=255) aGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTAcc > 1:114885/1‑89 (MQ=255) aGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACa > 1:100086/1‑90 (MQ=255) aTTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCAc > 2:562610/1‑90 (MQ=255) taaaTCCCGCCAAAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCAcc < 1:463020/88‑1 (MQ=255) | CATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACC > NZ_CP009273/1500197‑1500369 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |