| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,380,341 | 0 | G | A | 60.0% | ‑2.8 / 14.0 | 15 | intergenic (+31/‑72) | yfbL/yfbM | M28 family metallopeptidase/YfbM family protein |
| Reads supporting (aligned to +/- strand): ref base G (0/6); new base A (9/0); total (9/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.00e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.74e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCAC‑ATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGC > NZ_CP009273/2380277‑2380427 | tGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAAAGACAAGCAC‑AATTTTTTTTTCCTc > 2:95613/1‑90 (MQ=255) ggTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAAAGGCAAGCACAATTTTTTTTTccccc > 2:200545/1‑88 (MQ=255) tgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCAC‑ATTTTATTTTCCCTCATc < 1:127161/90‑1 (MQ=255) tgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCAC‑ATTTTATTTTCCCTCATc < 1:551668/90‑1 (MQ=255) tgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCAC‑ATTTTATTTTCCCTCATc < 2:711639/90‑1 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTTCCGAAAAAAAAACAAGGAA‑ATTTTTTTTTTTCTCATCTGtttttaa > 1:593731/1‑87 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCCGAAAAAAAGACAAGCAA‑ATTTTTTTTTTCCCCCTCtttttttaa > 2:134724/1‑87 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCCGAAAAAAAGAAAAGGAC‑AATTTTTTTTTTCTTATCtttttttaa > 1:526490/1‑87 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCCGAAAAAAAAAAAAGCAC‑AATTTTTTTTTTCTCCTCtttttttta > 1:706003/1‑87 (MQ=255) acaacaGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCAC‑ATTTTATTTTCCCTCATCTGTTTTAAGATGCGaaa < 2:310481/90‑1 (MQ=255) caGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAAAGACATGCAC‑ATTTTTTTTTTCCTCCTCTTTTTTAAAAAGCGgaaaaaa > 2:97224/1‑86 (MQ=255) ataaAAATTTCTCGAGGCGGGGTCAGAAAAAACGACACGCAC‑AATTTTTTTTTCCCCCTCTTTTTTAAAATGCGAAAAAAAAAAATCAAc > 2:221810/1‑90 (MQ=255) aaTTTCTCGAGGCGGGGCCAGAAAAAAAGACATGCAC‑ATTTTTTTTTTCCTCCTCTGTTTTTAAATGGGAAAAAAAAAAATTAAaggagg > 2:680857/1‑90 (MQ=255) gAGGCGGGTCCAGAAAAAGCGACATGCAC‑ATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAaaataa < 1:278388/90‑1 (MQ=255) aaaGCGACATGCAC‑ATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGc < 2:259260/90‑1 (MQ=255) | TGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCAC‑ATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGC > NZ_CP009273/2380277‑2380427 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |