| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,020,300 | 0 | A | C | 71.4% | ‑0.4 / 11.2 | 21 | E391D (GAA→GAC) | guaD | guanine deaminase |
| Reads supporting (aligned to +/- strand): ref base A (5/1); new base C (0/15); total (5/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.95e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.49e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAATTGTTCGTGATGATGACGTT > NZ_CP009273/3020214‑3020380 | aCGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAAccc > 2:627000/1‑90 (MQ=255) aTCTCTGGGCCTTGACGATTTGATTGCCACTTTTTTCCCGGGAAAAGAGGCTGATTTGGTGGTGAGGAACCCCACCGCCACTCCGCTAca < 1:577848/90‑1 (MQ=255) ctGGGCTTTGACGATTTGATTGGCAATTTTTTCCCGGGCAAAGGGGCTGATTTCGTGGTGATGGACCCCACCGCCACTCCGCTAcagcag < 1:80009/90‑1 (MQ=255) gggCTTTGACGTTTTGATTGGCAATTTTTTCCCTGGAAAAGAGGCTGATTTCGTGGTGAGGGACCCCACCGCCACTCCGCTACAGCAGCt < 2:387342/90‑1 (MQ=255) gggCCTTGACGTTTTGATTGGCAACTTTTTCCCTGGCAAAGAGGCTGATTTGGTGGTGATGGACCCCACCGCCACTCCGCTACAGCAGCt < 2:503874/90‑1 (MQ=255) ttGACGTTTTGATGGCCAATTTTTTACCTGACAAAGAGGTTGATTTCGTGGTGCGGGACCCCCCCGCCACTCCGCTACAGCAGCTGCGCt < 2:496563/90‑1 (MQ=255) gttttGATGGCCAATTTTTTCCCGGGCAAAGAGGCTGTTTTCGTGGTGATGGACCCCCCCGCCACTCCGCTACAGCAGCTGCGCTATGAc < 1:781760/88‑1 (MQ=255) ttttGCTAGGCAAATTTTTGCTGGGAAAAGAGGTTGTTTCCGGGGACAGGAACCCCTCCGCCACTCCGCTACAGCAGCTGCGCTATGACa < 1:714159/89‑1 (MQ=255) aTTTGATTGGCATTTTTTTCCCGGGAAAAGGGGCTGTTTTCGGGGTGAGGGACCCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACa < 1:340510/90‑1 (MQ=255) tGATTGACACTTTTTCCCCGGGAAAAGGGGCTGTTTTTGTGGTTAAGAAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAAct < 1:751820/90‑1 (MQ=255) gATTGCCAACTTTTTCCCTGGAAAAGGGGTTGTTTTGGGGGTGATGGACCCCCCCGCCACTCCGCTACAGCAGCTGCGCTATGACAActc < 1:776980/90‑1 (MQ=255) ttttACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAg > 1:358807/1‑90 (MQ=255) ttttACCTGGCAAAGAGGCTGATTTCGTGGGGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAg > 1:388880/1‑90 (MQ=255) tttCCCGGGAAAAGAGGCTGTTTTGGGGGTGATGAACCCCCCCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGt < 1:462872/90‑1 (MQ=255) ggCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACaaa > 2:267006/1‑90 (MQ=255) ttttGGTGGTGATGGACCCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAATTGTTCGtgatga < 2:358807/89‑1 (MQ=255) ttttCGGGGTGAGGAACCCCCCCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAATTGTTCGtgatga < 2:243731/89‑1 (MQ=255) tttGGTGGTGAGGAACCCCCCCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAATTGTTCGtgatgat < 1:556167/90‑1 (MQ=255) tCGGGGTGATGGACCCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAATTGTTCGtgatgatga < 1:26578/90‑1 (MQ=255) gtggtgATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAATTGTTCGTGATGATGACg > 2:81888/1‑90 (MQ=255) ggtgATGAACCCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAATTGTTCGTGATGATGACGtt < 1:122405/90‑1 (MQ=255) | ACGCTCGGCGGAGCGAAATCTCTGGGCCTTGACGATTTGATTGGCAACTTTTTACCTGGCAAAGAGGCTGATTTCGTGGTGATGGAACCCACCGCCACTCCGCTACAGCAGCTGCGCTATGACAACTCTGTTTCTTTAGTCGACAAATTGTTCGTGATGATGACGTT > NZ_CP009273/3020214‑3020380 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |