| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 50,279 | T→C | F153L (TTT→CTT) | folA → | type 3 dihydrofolate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 50,279 | 0 | T | C | 100.0% | 57.6 / NA | 16 | F153L (TTT→CTT) | folA | type 3 dihydrofolate reductase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (7/9); total (7/9) | |||||||||||
ATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTTTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGCCTTCC > NZ_CP009273/50193‑50364 | aTTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCCTTg > 1:162507/1‑90 (MQ=255) ttCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCCTTgag < 2:687696/90‑1 (MQ=255) aCGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCCTTGAGATTCTGGAgc > 1:470711/1‑90 (MQ=255) aGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCCTTGAGATTCTGGAgcggc < 2:436733/90‑1 (MQ=255) gACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCCTTGAGATTCTGGAGCGGCGGTAAttt < 2:745119/90‑1 (MQ=255) gAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCCTTGAGATTCTGGAGCGGCGGTAATTTTGtata < 2:728409/90‑1 (MQ=255) cGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCCTTGAGATTCTGGAGCGGCGGTAATTTTTTATAGAATTTACGGCTAg > 1:281680/1‑90 (MQ=255) aaTTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCCTTGAGATTCTGGAGCGGCGGTAATTTTTTATAGAATTTACGGCTAacg > 2:429532/1‑90 (MQ=255) aaTTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCCTTGAGATTCTGGAGCGGCGGGAATTTTTTATAGAATTTTCCGCTaacc > 1:49666/1‑87 (MQ=255) aTGCTGATGCGCAGAACTCTCACAGCTATTGCCTTGAGATTCTGGAGCGGCGGTAATTTTTTATAGAATTTACGGCTAGCGCCGGATGCg > 2:752435/1‑90 (MQ=255) aTGCTGATGCGCAGAACTCTCACAGCTATTGCCTTGAGATTCTGGAGCGGCGGGAATTTTTTTTAAAATTTACGGCTAACGCCCGGTGCg > 1:573638/1‑90 (MQ=255) gCTATTGCCTTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGcc < 1:429532/90‑1 (MQ=255) gCTATTGCCTTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGcc < 2:491907/90‑1 (MQ=255) gCTATTGCCTTGAGATTCTGGAGCGGCGGTAATTTTGTAAAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGcc < 2:588148/90‑1 (MQ=255) ttGCCTTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGCCTTcc < 1:442034/90‑1 (MQ=255) ttGCCTTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGCCTTcc < 1:599130/90‑1 (MQ=255) | ATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTTTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGCCTTCC > NZ_CP009273/50193‑50364 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |