Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,613,723	G→C	G116A (GGC→GCC)	marR →	multiple antibiotic resistance transcriptional regulator MarR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,613,723	0	G	C	100.0%	107.3 / NA	29	G116A (GGC→GCC)	marR	multiple antibiotic resistance transcriptional regulator MarR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (10/19);  total (10/19)

TGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCG  >  NZ_CP009273/1613636‑1613808                                                                                        |                                                                                      tGCCGAACCCGAATGACAAGCGCGGCGTACTGGT‑AAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGCCCa                                                                                    >  2:750229/1‑90 (MQ=255)  gCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCGATCAATTAGTTGCCCa                                                                                    <  1:441635/90‑1 (MQ=255)     gAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGCCCAGGa                                                                                 >  1:97330/1‑90 (MQ=255)             aTGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGCCCAGGACCTGCAcc                                                                         <  1:470369/90‑1 (MQ=255)              tGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGCCCAGGACCTGCACCa                                                                        <  2:314108/90‑1 (MQ=255)               gACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGCCCAGGACCTGCACCaa                                                                       <  2:178262/90‑1 (MQ=255)                     cgcgGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGCCCAGGCCCTGCACCAAGAATTa                                                                 <  1:435342/90‑1 (MQ=255)                           gTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGCCCAGGACCTGCACCAAGAATTAACaaaa                                                           <  2:718267/90‑1 (MQ=255)                           gTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGCCCAGGACCTGCACCAAGAATTAACaaaa                                                           <  2:114348/90‑1 (MQ=255)                                        ttACCACCGGCGGCGCGGCAATATGTGAGCAATGCCATCAATTAGTTGCCCAGGACCTGCACCAAGAATTAACAAAAAACCTGAcggcgg                                              <  1:427197/90‑1 (MQ=255)                                        ttACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGCCCAGGACGTGCACCAAGAATTAACAAAAAACCTGAcggcgg                                              >  2:447709/1‑90 (MQ=255)                                        ttACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGCCCAGGACCTGCACCAAGAATTAACAAAAAACCTGAcggcgg                                              >  2:512722/1‑90 (MQ=255)                                                ggcggcGCGGCAATATGTGAACAATGCCATCAATTAGTTGCCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTg                                      <  2:220818/90‑1 (MQ=255)                                                ggcggcGCGGCAATATGTGAACAATGCCATCAATTAGTTGCCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTg                                      >  2:148466/1‑90 (MQ=255)                                                  cggcgcggcAATATGTGAACAATGCCATCAATTAGTTGCCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGc                                    <  2:680311/90‑1 (MQ=255)                                                     cgcggcAATATGTGAACAATGCCATCAATTAGTTGCCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAac                                 <  1:633822/90‑1 (MQ=255)                                                     cgcggcAATATGTGAACAATGCCATCAATTAGTTGCCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAac                                 <  1:659997/90‑1 (MQ=255)                                                                gtgAACAATGCCATCAATTAGTTGCCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACTAAGTGGCAACACTTGAGTAtt                      <  1:750229/90‑1 (MQ=255)                                                                gtgAACAATGCCATCAATTAGTTGCCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTAtt                      <  1:52336/90‑1 (MQ=255)                                                                      aaTGCCATCAATTAGTTGCCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTa                >  2:420989/1‑90 (MQ=255)                                                                      aaTGCCATCAATTAGTTGCCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTa                >  2:496251/1‑90 (MQ=255)                                                                       aTGCCATCAATTAGTTGCCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTaa               >  1:359052/1‑90 (MQ=255)                                                                       aTGCCATCAATTAGTTGCCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTaa               >  2:468466/1‑90 (MQ=255)                                                                        tGCCATCAATTAGTTGCCCAGGACCTGCACCAAGAATTAACAAAAAACCTGAcggcg                                               <  2:112446/57‑1 (MQ=255)                                                                        tGCCATCAATTAGTTGCCCAGGACCTGCACCAAGAATTAACAAAAAACCTGAcggcg                                               >  1:112446/1‑57 (MQ=255)                                                                          ccATCAATTAGTTGCCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGaa            <  1:427227/90‑1 (MQ=255)                                                                          ccATCAATTAGTTGCCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGaa            <  1:326401/90‑1 (MQ=255)                                                                               aaTTAGTTGCCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTcc       <  1:148466/90‑1 (MQ=255)                                                                                    gTTGCCCAGGCCCTGCACCAAAAATTAAAAAAAAACCTGACGGCAGACGAAGCGGCAACGCTTGAGTCTTTGCTTAAGAAAGTCCTGCCg  <  2:6342/90‑1 (MQ=255)                                                                                        |                                                                                      TGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCG  >  NZ_CP009273/1613636‑1613808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑