Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273510,0800AC54.5% 8.1 / 11.4 11V79G (GTG→GGG) ybbJNfeD family protein
Reads supporting (aligned to +/- strand):  ref base A (3/2);  new base C (0/6);  total (3/8)
Fisher's exact test for biased strand distribution p-value = 6.06e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.26e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

AGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCC  >  NZ_CP009273/509996‑510146
                                                                                    |                                                                  
aGCGGAGATTCCAGCACAAAGCGCCGGCCAATCAGCTGCCCCCCGCGTTGGTTAAAATGACTATCGCTTTGTTTTTGTTCGCGCCCccgc                                                               <  1:885501/90‑1 (MQ=255)
           caccacaAATCGCCGGCCAATCGGCTGCTGCCCGCGTTGTTTTAAATGACTCCCGTTGTGTTTTTCTGCGCTCCCCCGCCGCGACAACCa                                                    <  2:811514/87‑1 (MQ=255)
                   aaCGTCGGCCAATCAGCTGCTCCCCGCGCTGTTTTAAATGACTATCGTTGTGTTTTTGTCCGCGCCCCCGCCGCGACAACCATTTccacc                                            <  1:398190/90‑1 (MQ=255)
                                    tgctccCCGCGCTGTTTAAAATCACTACCGCTGTTTTTTTGTCCGCCCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGagc                           <  1:117092/90‑1 (MQ=255)
                                                   ttaaaaTAACTATCGTTTTGTTTTTTTCCGCGCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCa            <  1:399397/87‑1 (MQ=255)
                                                                   tgtgCTTTTGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGagc                           <  1:489907/59‑1 (MQ=255)
                                                                   tgtgCTTTTGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGagc                           >  2:489907/1‑59 (MQ=255)
                                                                              tCGCGCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTAccc  <  1:196287/73‑1 (MQ=255)
                                                                              tCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTAccc  >  2:196287/1‑73 (MQ=255)
                                                                                gcgcACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGagc                           <  1:795672/46‑1 (MQ=255)
                                                                                gcgcACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGagc                           >  2:795672/1‑46 (MQ=255)
                                                                                    |                                                                  
AGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCC  >  NZ_CP009273/509996‑510146

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑