| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,429,984 | 0 | G | A | 50.0% | 7.5 / 12.2 | 18 | intergenic (‑108/+33) | uspF/ompN | universal stress protein UspF/porin OmpN |
| Reads supporting (aligned to +/- strand): ref base G (9/0); new base A (0/9); total (9/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.11e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGG > NZ_CP009273/1429902‑1430072 | aaaaGTGAAACGCCAGATGACTTTTTTTGTGAAATGACGGAGTTCACTTTTTTATTTTAGAAAAAAGGAGAAAAAAAAAGAAAAAAAAGc < 1:151513/89‑1 (MQ=255) gtAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCa > 2:503031/1‑90 (MQ=255) tAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCAt > 1:60461/1‑90 (MQ=255) caaGATGACTTTTTTTGTGAAAGGACGGATTTCATTTTTTAAATTAAAAAAAAAGGAGAAAAAAAAAAAAAAAAAACCCCGCCATAACAg < 1:918346/88‑1 (MQ=255) aGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCg > 1:10974/1‑90 (MQ=255) cacTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCagg > 1:870517/1‑90 (MQ=255) ttttttGTGAAAGGAGGGATTTCACTTTTTTAATTAAAAAAAAAGGAGAAAAAAAAAGAAAAAAAACCCCGCCATAACAGCGGGCaggag < 1:88783/89‑1 (MQ=255) ttttttGTAAAATGAGGGATTTCACTTTTTAAATTTAGAAAAAAGGAGAAAAAAAAACAAAAAAAACCCCGCCATAACAGCGGGCaggag < 1:598057/89‑1 (MQ=255) ttttGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAggagga > 1:110704/1‑90 (MQ=255) ttttGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAggagga > 1:429048/1‑90 (MQ=255) ttCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCa > 1:169000/1‑90 (MQ=255) acaTTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGAc > 2:858483/1‑90 (MQ=255) tttAATTTAGAAAAAGGGAGAAAAAAAAAGAAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTaaa < 2:110704/90‑1 (MQ=255) tttAATTAAGAAAAAAGGAGAAAAAAAAAGAAAAAAAACCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTaaa < 2:405747/90‑1 (MQ=255) aagaagAAAAAAAAAGAAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATatcatc < 2:745284/90‑1 (MQ=255) aaGGAGGAAAAAAAAGCAAAAAAACCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATatcatc < 2:563500/90‑1 (MQ=255) aggagaAAAAATAACAAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCg < 2:904364/90‑1 (MQ=255) aGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTgg > 2:177748/1‑90 (MQ=255) | TAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGG > NZ_CP009273/1429902‑1430072 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |