| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,598,494 | 0 | C | T | 52.6% | 9.4 / 26.6 | 19 | S64L (TCG→TTG) | lsrD | autoinducer 2 ABC transporter permease LsrD |
| Reads supporting (aligned to +/- strand): ref base C (1/8); new base T (10/0); total (11/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.19e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.68e-05 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCT > NZ_CP009273/1598406‑1598578 | ttGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGGTCGAGATTTct > 1:7527/1‑89 (MQ=255) aGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGGTCGATATTTTGTTTTGTTTGACCCTc > 2:142470/1‑90 (MQ=255) cTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGGTCGATATTTTGTTTTGTTCGACCCTCCGCct > 2:268468/1‑90 (MQ=255) cTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTgcgc < 2:464774/90‑1 (MQ=255) gcattggcattgTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGGTCGGTATTTTTTTTTGTTCGGCCATCGGCCTCTGCGccc > 1:428049/1‑89 (MQ=255) ggcattgTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTTTTTTGGTTCGACCATCGGCCTCTTCGCcctttca > 2:228285/1‑90 (MQ=255) tgTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGGTTGATATTTTGTTTGGTTTGACCACCGGCCTCTGCGCCCTTGCCTTtgg > 1:572604/1‑90 (MQ=255) cccTACCGCTAACGATGGTGATTGTCAGTGGCGGGGGCGATATTTTGTTTTGTTTGGCCCTCCGCCCCCGCGCCCTTGCCTTTGGCGtgc > 1:10270/1‑88 (MQ=255) cccTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTTGTTTTGTTTGACCCTCGGCCTCTGCGCCCTTGCATTTGGGGTAc > 2:421845/1‑90 (MQ=255) cccTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTTGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGGAc > 2:303647/1‑90 (MQ=255) cccTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTAc < 2:572604/90‑1 (MQ=255) cccTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTAc < 1:421845/90‑1 (MQ=255) cccTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTAc < 2:128226/90‑1 (MQ=255) cGCTAACGATGGTGATTGTCAGTGGCGGGGTCGGTATTTTGTTTTGTTTGACCACCGGCCCCTTCGCCCTTTCCTTTGGGGGGCTGtttt > 1:247869/1‑89 (MQ=255) tGGTGATTGTCAGTGGCGGGGTCGATATTTTTTTTTGTTTGACCCCCGGCCCCCTCGCCCTTTCATTTGGGGGACTGTTTTTAAatggtg > 1:809475/1‑90 (MQ=255) gTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGc < 2:809475/90‑1 (MQ=255) ggATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGa < 1:482823/90‑1 (MQ=255) gATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGAt < 1:930604/90‑1 (MQ=255) cGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACt > 2:746021/1‑90 (MQ=255) tatTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCt < 1:268468/90‑1 (MQ=255) | TTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCT > NZ_CP009273/1598406‑1598578 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |