| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,663,765 | 0 | T | A | 64.3% | 0.3 / 12.0 | 14 | I29F (ATT→TTT) | ynfL | LysR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (0/5); new base A (9/0); total (9/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.00e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.61e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTGCGATTGGTTCGTGCCAGCAGTCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAAT > NZ_CP009273/1663683‑1663843 | cTGCGATTGGTTCGTGCCAGCAGTCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGGAAAATTTAgg > 2:141886/1‑90 (MQ=255)cTGCGATTGGTTCGTGCCAGCAGTCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGGTGGGGAAAATTTAgg > 2:646666/1‑90 (MQ=255)cTGCGATTGGTTCGTGCCAGCAGTCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGGTGCGAAAAAtttaag > 1:320866/1‑86 (MQ=255) gCGATTGGTTCGTGCCAGCAGTCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGGGAAAAATTTAgggc > 2:375345/1‑88 (MQ=255) aTTGGTTCGTGCCAGCAGTCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGGAAAATTCAggggggg > 2:855049/1‑89 (MQ=255) aTTGGTTCGTGCCAGCAGTCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAAAATTTAggggggg > 2:921982/1‑89 (MQ=255) cGTGCCAGCAGTCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTGACGGAAAATTCAAGGGGGCAgccgtg > 2:870783/1‑86 (MQ=255) tGCCAGCAGTCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGgcgcg < 2:879354/90‑1 (MQ=255) gcagTCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGa < 1:925575/90‑1 (MQ=255) ttgCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCgcaac < 2:853245/90‑1 (MQ=255) ccAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAAAATTCAGGCGGGCAGCGGCGCGCCCCAAAAGCAGCTCTTCCgcaacagcaa > 2:332580/1‑90 (MQ=255) cTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGa < 1:72228/90‑1 (MQ=255) cTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGa < 1:870783/90‑1 (MQ=255) cggTTGCGAAAAATTTAGGGGGGCAGCGGCGCGCCCCCAAAGCAGCTCTTTCCCAACAACAAAAAAAAAAAACAGATGACGAAGATTAat > 1:61333/1‑90 (MQ=255) | CTGCGATTGGTTCGTGCCAGCAGTCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAAT > NZ_CP009273/1663683‑1663843 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |