Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,738,4480AC59.3% 18.9 / 30.3 27V84G (GTG→GGG) trmDtRNA (guanosine(37)‑N1)‑methyltransferase TrmD
Reads supporting (aligned to +/- strand):  ref base A (8/3);  new base C (0/16);  total (8/19)
Fisher's exact test for biased strand distribution p-value = 7.43e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.09e-05
Rejected as consensus: Frequency below/above cutoff threshold.

GACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCG  >  NZ_CP009273/2738359‑2738537
                                                                                         |                                                                                         
gACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCa                                                                                           <  2:804364/90‑1 (MQ=255)
gACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCa                                                                                           >  2:640588/1‑90 (MQ=255)
gACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCa                                                                                           >  2:553781/1‑90 (MQ=255)
gACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCa                                                                                           >  1:836179/1‑90 (MQ=255)
         ccAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTgcgc                                                                                  <  1:86162/90‑1 (MQ=255)
                 aaTTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCAccc                                                                          >  2:932193/1‑90 (MQ=255)
                                gCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCACCCGCCGCGGCTTTtgct                                                           <  2:28188/90‑1 (MQ=255)
                                gCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCACCCGCCGCGGCTTTtgct                                                           <  2:388876/90‑1 (MQ=255)
                                gCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGCCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTtgct                                                           <  1:172186/90‑1 (MQ=255)
                                   aGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTg                                                                                     <  1:761599/61‑1 (MQ=255)
                                   aGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTg                                                                                     >  2:761599/1‑61 (MQ=255)
                                        gCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAAt                                                   >  1:661469/1‑90 (MQ=255)
                                          tGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATgg                                                 <  2:175015/90‑1 (MQ=255)
                                              gCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTc                                             <  2:345566/90‑1 (MQ=255)
                                              gCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTc                                             <  1:932193/90‑1 (MQ=255)
                                                   cTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCTTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCa                                        <  1:502816/90‑1 (MQ=255)
                                                   cTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCa                                        <  2:919871/90‑1 (MQ=255)
                                                   cTTGATCAACCTTGCGTCCATGTGGTGACAGATAAATCCCCTTTGCCCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCa                                        <  2:281812/90‑1 (MQ=255)
                                                         cAAGCTTGCGCCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGtt                                  <  2:911611/90‑1 (MQ=255)
                                                          aaGCTTGCGTCCTTGTGGTGACAGAAAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTg                                 <  1:553781/90‑1 (MQ=255)
                                                           aGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGc                                >  1:868398/1‑90 (MQ=255)
                                                             cTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCAc                              <  2:836179/90‑1 (MQ=255)
                                                             cTTGCGTCCCTGTGGTGACAGAAAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCAc                              <  1:302772/90‑1 (MQ=255)
                                                              ttGCGTCCTTGTGGTGACAGATAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCAcc                             <  2:868398/90‑1 (MQ=255)
                                                              ttGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCAcc                             <  2:909747/90‑1 (MQ=255)
                                                                gCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACcat                           >  1:763852/1‑90 (MQ=255)
                                                                         tggtgACAGAAAAACCCCCTTTGCCCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACAt                  <  1:657368/90‑1 (MQ=255)
                                                                             gACAGATAAATCCCCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATcccc              <  2:833167/90‑1 (MQ=255)
                                                                             gACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATcccc              >  2:672864/1‑90 (MQ=255)
                                                                             gACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATcccc              >  2:258063/1‑90 (MQ=255)
                                                                                  aTAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTcc         >  2:631701/1‑90 (MQ=255)
                                                                                         aCCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTccgccgccg  >  1:538243/1‑90 (MQ=255)
                                                                                         |                                                                                         
GACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCG  >  NZ_CP009273/2738359‑2738537

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: