| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,175,957 | 0 | A | T | 51.8% | 13.9 / 32.3 | 27 | I17F (ATT→TTT) | zupT | zinc transporter ZupT |
| Reads supporting (aligned to +/- strand): ref base A (5/8); new base T (14/0); total (19/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.80e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.60e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTC > NZ_CP009273/3175872‑3176046 | gATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTgg < 2:634173/90‑1 (MQ=255) tCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGAGCCACGGTTTTTTgcg > 2:369641/1‑90 (MQ=255) gAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTTTTTGCGCGTTTTTCGGCGt > 2:846032/1‑90 (MQ=255) gAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGACGGCACGGTTTTTGGTGCTTTTCTCgtggg > 2:904677/1‑86 (MQ=255) aTTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACCTTTTTTGGGGCGGTTTTCCGCGGtctc > 2:561173/1‑90 (MQ=255) gAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGGGCCCCGTTTTTTTGGGCGGTTTTCTGCCGTCTCCGGGgaaaa > 1:564610/1‑90 (MQ=255) aGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGAGCCACGGTTTTTTGCGCGTTTTTCCGCGTTCTCCGGCaaaaaa > 2:926506/1‑89 (MQ=255) gTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGGGCCCCGGTTTTTTGCGCGTTTTTTTGCGTTCTCTTGCGAAAAcc > 1:757529/1‑90 (MQ=255) gTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGAGCCACGTTTATTGGCGCGTTTTTCGGCGTTCTCGGGCaaaaaac > 2:332729/1‑88 (MQ=255) gTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCCCGTTTATTGGCGCGTTTTTCGGCGTTCTCGGGGaaaaaac > 2:360944/1‑88 (MQ=255) gTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTTTTGGCGCGTTTCTCGGCGTTCTCGGGCaaaaaac > 2:755974/1‑88 (MQ=255) ttgATGTCAGTACCTCTCATTCTGGCCATACTGGCGGGGGCAGCCACGTTTATTGGCGGGTTTCTCGGCGGTCGCGGGCCAAAACCCTCg < 1:340460/88‑1 (MQ=255) aTGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAAc < 2:603127/90‑1 (MQ=255) gTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTTTTGGGGCGTTTTTCGGCGTTCTCGGGCAAAAAACCTCGAACCCCTTa > 2:485366/1‑90 (MQ=255) ctctCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTgg < 1:360944/90‑1 (MQ=255) tctcATTCTGACCATACTGGCGGGGGGAGCCACGTTTATTTGGGCGGTTTTCGGCGTTCTCGGGCAAAAAACCCCCAACCGCCTACTGGc > 2:862138/1‑90 (MQ=255) tctcATTCTGACCATACTGGCGGGGGCAGCCCCGTTTTTTTGCGCGGTTCTCGGCGGTCTCCGGCAAAAAACCCCCAACCCCTTTCTggg > 1:196719/1‑89 (MQ=255) tctcATTCTGACCATACTGGCGGGGGCAGCCACGTTTTTTGGGGCGTTTTTCGGCGTTTTCGGGGAAAAACCCTCGAACCCCTTACTTGc > 2:564245/1‑90 (MQ=255) gACCATACTGGCGGGGGGAGCCCCGGTTATTTGCGCGGTTTTCGCCGTTCTCGGGGAAAAAACCCCGAACCCCATCTTCGCGTTTTCGCt > 1:920240/1‑90 (MQ=255) gACCATACTGGCGGGGGGAGCCACGTTTTTTTGCGCGGTTTTCGGCGTTCTCGGGCAAAAAACCTCGAACCGCTTTCTGGGGGTTTTGCt > 2:487379/1‑90 (MQ=255) aTACTGGCGGGGGCAGCCACGTTTTTTTGCGCGTTTTTCGGCGTTCTCGGGCAAAAACCCTCGAACCGCTTTCTGGCGTTTTTGCTAggg > 2:396562/1‑89 (MQ=255) gCGGGGGCAGCCCCGTTTTTTTGCGCGTTTCTCCGCGTTCTCGGGCAAAAAACCCCCAACCCCCTTCTGGCGGTTTTGCTAGGGTTTtcg > 1:66187/1‑90 (MQ=255) ggggCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTgcggcg < 2:674960/90‑1 (MQ=255) ggggCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTgcggcg < 1:926506/90‑1 (MQ=255) ggggCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTgcggcg < 1:872835/90‑1 (MQ=255) ggggCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTgcggcg < 1:786987/90‑1 (MQ=255) cAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCggggg > 2:415732/1‑89 (MQ=255) aTTGGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGctc < 1:561173/90‑1 (MQ=255) aTTGGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGctc < 1:755974/90‑1 (MQ=255) | GATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTC > NZ_CP009273/3175872‑3176046 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |